Cell death by polyvinylpyrrolidine-coated silver nanoparticles is mediated by ROS-dependent signaling

Kyeongah Kang; Hyeyoun Jung; Jong Seok Lim

doi:10.4062/biomolther.2012.20.4.399

Cell death by polyvinylpyrrolidine-coated silver nanoparticles is mediated by ROS-dependent signaling

Kyeongah Kang, Hyeyoun Jung, Jong Seok Lim

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Silver nanoparticles (AgNPs) are widely used nanoparticles and they are mainly used in antibacterial and personal care products. In this study, we evaluated the effect of AgNPs on cell death induction in the murine dendritic cell line DC2.4. DC2.4 cells exposed to AgNPs showed a marked decrease in cell viability and an induction of lactate dehydrogenase (LDH) leakage in a time- and dose-dependent manner. In addition, AgNPs promoted reactive oxygen species (ROS)-dependent apoptosis and AgNP-induced ROS triggered a decrease in mitochondrial membrane potential. The activation of the intracellular signal transduction pathway was also observed in cells cultured with AgNPs. Taken together, our data demonstrate that AgNPs are able to induce a cytotoxic effect in DCs through ROS generation. This study provides important information about the safety of AgNPs that may help in guiding the development of nanotechnology applications.

Original language	English
Pages (from-to)	399-405
Number of pages	7
Journal	Biomolecules and Therapeutics
Volume	20
Issue number	4
DOIs	https://doi.org/10.4062/biomolther.2012.20.4.399
State	Published - 2012
Externally published	Yes

Keywords

Apoptosis
Dendritic cells
Intracellular signaling
Reactive oxygen species (ROS)
Silver nanoparticle

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10.4062/biomolther.2012.20.4.399

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title = "Cell death by polyvinylpyrrolidine-coated silver nanoparticles is mediated by ROS-dependent signaling",

abstract = "Silver nanoparticles (AgNPs) are widely used nanoparticles and they are mainly used in antibacterial and personal care products. In this study, we evaluated the effect of AgNPs on cell death induction in the murine dendritic cell line DC2.4. DC2.4 cells exposed to AgNPs showed a marked decrease in cell viability and an induction of lactate dehydrogenase (LDH) leakage in a time- and dose-dependent manner. In addition, AgNPs promoted reactive oxygen species (ROS)-dependent apoptosis and AgNP-induced ROS triggered a decrease in mitochondrial membrane potential. The activation of the intracellular signal transduction pathway was also observed in cells cultured with AgNPs. Taken together, our data demonstrate that AgNPs are able to induce a cytotoxic effect in DCs through ROS generation. This study provides important information about the safety of AgNPs that may help in guiding the development of nanotechnology applications.",

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T1 - Cell death by polyvinylpyrrolidine-coated silver nanoparticles is mediated by ROS-dependent signaling

AU - Kang, Kyeongah

AU - Jung, Hyeyoun

AU - Lim, Jong Seok

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N2 - Silver nanoparticles (AgNPs) are widely used nanoparticles and they are mainly used in antibacterial and personal care products. In this study, we evaluated the effect of AgNPs on cell death induction in the murine dendritic cell line DC2.4. DC2.4 cells exposed to AgNPs showed a marked decrease in cell viability and an induction of lactate dehydrogenase (LDH) leakage in a time- and dose-dependent manner. In addition, AgNPs promoted reactive oxygen species (ROS)-dependent apoptosis and AgNP-induced ROS triggered a decrease in mitochondrial membrane potential. The activation of the intracellular signal transduction pathway was also observed in cells cultured with AgNPs. Taken together, our data demonstrate that AgNPs are able to induce a cytotoxic effect in DCs through ROS generation. This study provides important information about the safety of AgNPs that may help in guiding the development of nanotechnology applications.

AB - Silver nanoparticles (AgNPs) are widely used nanoparticles and they are mainly used in antibacterial and personal care products. In this study, we evaluated the effect of AgNPs on cell death induction in the murine dendritic cell line DC2.4. DC2.4 cells exposed to AgNPs showed a marked decrease in cell viability and an induction of lactate dehydrogenase (LDH) leakage in a time- and dose-dependent manner. In addition, AgNPs promoted reactive oxygen species (ROS)-dependent apoptosis and AgNP-induced ROS triggered a decrease in mitochondrial membrane potential. The activation of the intracellular signal transduction pathway was also observed in cells cultured with AgNPs. Taken together, our data demonstrate that AgNPs are able to induce a cytotoxic effect in DCs through ROS generation. This study provides important information about the safety of AgNPs that may help in guiding the development of nanotechnology applications.

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KW - Dendritic cells

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Cell death by polyvinylpyrrolidine-coated silver nanoparticles is mediated by ROS-dependent signaling

Abstract

Keywords

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