Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood

S. M. Lipson, A. B. Ashraf, S. H. Lee, M. H. Kaplan, D. H. Shepp

Research output: Contribution to journalComment/debate

12 Scopus citations

Abstract

We determined the efficacy of PCR for the direct detection of infectious cytomegalovirus (CMV) in specimen-inoculated MRC-5 tube cultures (cell culture-PCR [CC-PCR]). Parallel testing was performed by a shell vial assay- indirect immunofluorescence assay (SVA-IFA) and isolation (conventional MRC tube cultures [TC-CPE]. The sensitivity, specificity, and positive and negative predictive values for CC-PCR, SVA-IFA, and TC-CPE were 81, 99, 94, and 94%, 86, 100, 100, and 96%, and 77, 100, 100, and 93%, respectively. Future application of CC-PCR may be directed toward its use as a screening tool for cytomegalovirus genotypic variants.

Original languageEnglish
Pages (from-to)1411-1413
Number of pages3
JournalJournal of Clinical Microbiology
Volume33
Issue number5
DOIs
StatePublished - 1995
Externally publishedYes

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