TY - JOUR
T1 - Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood
AU - Lipson, S. M.
AU - Ashraf, A. B.
AU - Lee, S. H.
AU - Kaplan, M. H.
AU - Shepp, D. H.
PY - 1995
Y1 - 1995
N2 - We determined the efficacy of PCR for the direct detection of infectious cytomegalovirus (CMV) in specimen-inoculated MRC-5 tube cultures (cell culture-PCR [CC-PCR]). Parallel testing was performed by a shell vial assay- indirect immunofluorescence assay (SVA-IFA) and isolation (conventional MRC tube cultures [TC-CPE]. The sensitivity, specificity, and positive and negative predictive values for CC-PCR, SVA-IFA, and TC-CPE were 81, 99, 94, and 94%, 86, 100, 100, and 96%, and 77, 100, 100, and 93%, respectively. Future application of CC-PCR may be directed toward its use as a screening tool for cytomegalovirus genotypic variants.
AB - We determined the efficacy of PCR for the direct detection of infectious cytomegalovirus (CMV) in specimen-inoculated MRC-5 tube cultures (cell culture-PCR [CC-PCR]). Parallel testing was performed by a shell vial assay- indirect immunofluorescence assay (SVA-IFA) and isolation (conventional MRC tube cultures [TC-CPE]. The sensitivity, specificity, and positive and negative predictive values for CC-PCR, SVA-IFA, and TC-CPE were 81, 99, 94, and 94%, 86, 100, 100, and 96%, and 77, 100, 100, and 93%, respectively. Future application of CC-PCR may be directed toward its use as a screening tool for cytomegalovirus genotypic variants.
UR - http://www.scopus.com/inward/record.url?scp=0028969446&partnerID=8YFLogxK
U2 - 10.1128/jcm.33.5.1411-1413.1995
DO - 10.1128/jcm.33.5.1411-1413.1995
M3 - Comment/debate
C2 - 7615769
AN - SCOPUS:0028969446
SN - 0095-1137
VL - 33
SP - 1411
EP - 1413
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 5
ER -