CD22-mediated stimulation of T cells regulates T-cell receptor/CDS-induced signaling

Alejandro Aruffo, Steven B. Kanner, Dennis Sgroi, Jeffrey A. Ledbetter, Ivan Stamenkovic

Research output: Contribution to journalArticlepeer-review

99 Scopus citations

Abstract

Interaction between B lymphocytes and other cell types is mediated in part by the B-cell adhesion molecule CD22. Recent work has suggested one of the T-cell ligands of B cells to be CD45RO, an isoform of the receptor-linked phosphotyrosine phosphatase CD45. Here we demonstrate direct interaction between CD22 and several isoforms of CD45, including CD45RO, and propose that the interaction may participate in regulation of lymphocyte signaling. Cross-linking of CD3 and CD22 T-cell ligands with anti-CD3 antibody and soluble CD22 is shown to block anti-CD3-induced intracellular calcium increase and to inhibit tyrosine phosphorylation of phospholipase Cγ1. These effects are consistent with those observed upon coligation of CD3 and CD45 with antibody, providing support to the possibility that ligand-mediated stimulation of CD45 may result in modulation of substrate phosphorylation and lymphocyte activation.

Original languageEnglish
Pages (from-to)10242-10246
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number21
StatePublished - 1992
Externally publishedYes

Keywords

  • Adhesion
  • CD22
  • CD45
  • Carbohydrate
  • Sialic acid

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