@article{4088b7d5e9a04406900bd84ba8fe56fd,
title = "CD169 + macrophages provide a niche promoting erythropoiesis under homeostasis and stress",
abstract = "A role for macrophages in erythropoiesis was suggested several decades ago when erythroblastic islands in the bone marrow, composed of a central macrophage surrounded by developing erythroblasts, were described. However, the in vivo role of macrophages in erythropoiesis under homeostatic conditions or in disease remains unclear. We found that specific depletion of CD169 + macrophages markedly reduced the number of erythroblasts in the bone marrow but did not result in overt anemia under homeostatic conditions, probably because of concomitant alterations in red blood cell clearance. However, CD169 + macrophage depletion significantly impaired erythropoietic recovery from hemolytic anemia, acute blood loss and myeloablation. Furthermore, macrophage depletion normalized the erythroid compartment in a JAK2 V617F-driven mouse model of polycythemia vera, suggesting that erythropoiesis in polycythemia vera remains under the control of macrophages in the bone marrow and splenic microenvironments. These results indicate that CD169 + macrophages promote late erythroid maturation and that modulation of the macrophage compartment may be a new strategy to treat erythropoietic disorders.",
author = "Andrew Chow and Matthew Huggins and Jalal Ahmed and Daigo Hashimoto and Daniel Lucas and Yuya Kunisaki and Sandra Pinho and Marylene Leboeuf and Clara Noizat and {Van Rooijen}, Nico and Masato Tanaka and Zhao, {Zhizhuang Joe} and Aviv Bergman and Miriam Merad and Frenette, {Paul S.}",
note = "Funding Information: We thank C. Prophete, N. Dholakia, the Mount Sinai School of Medicine (MSSM) Microscopy Shared Resource Facility, J. Qi and S. Kim-Schulze at the MSSM Human Immune Monitoring Center, J. Ochando and X. Qiao at the MSSM Flow Cytometry Shared Resource Center and L. Tesfa at the Albert Einstein College of Medicine Flow Cytometry Sorting Facility for providing reagents, technical assistance and guidance. This work was supported by US National Institutes of Health grants (R01 grants HL097700, HL069438 and DK056638 to P.S.F., R01CA112100 to M.M. and R01HL116340 to P.S.F. and M.M.). A.C., J.A., D.L. and Y.K. were supported by fellowships from the National Heart, Lung and Blood Institute (5F30HL099028, A.C.), the National Institute of General Medical Sciences (T32GM062754, J.A.), Fundaci{\'o}n Ram{\'o}n Areces (D.L.) and the Japan Society for the Promotion of Science (Y.K.).",
year = "2013",
month = apr,
doi = "10.1038/nm.3057",
language = "English",
volume = "19",
pages = "429--436",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "4",
}