Cardioprotection by minocycline in a rabbit model of ischemia/reperfusion injury: Detection of cell death by in vivo 111In-GSAO SPECT

Takayoshi Yamaki; Hans J. de Haas; Nobuhiro Tahara; Artiom Petrov; Dilbahar Mohar; Nezam Haider; Jun Zhou; Atsuko Tahara; Yasuchika Takeishi; Hendrikus H. Boersma; Tiziano Scarabelli; Annapoorna Kini; H. William Strauss; Jagat Narula

doi:10.1007/s12350-017-1031-8

Cardioprotection by minocycline in a rabbit model of ischemia/reperfusion injury: Detection of cell death by in vivo ¹¹¹In-GSAO SPECT

Takayoshi Yamaki, Hans J. de Haas, Nobuhiro Tahara, Artiom Petrov, Dilbahar Mohar, Nezam Haider, Jun Zhou, Atsuko Tahara, Yasuchika Takeishi, Hendrikus H. Boersma, Tiziano Scarabelli, Annapoorna Kini, H. William Strauss, Jagat Narula

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Background: Preclinical studies indicate that minocycline protects against myocardial ischemia/reperfusion injury. In these studies, minocycline was administered before ischemia, which can rarely occur in clinical practice. The current study aimed to evaluate cardioprotection by minocycline treatment upon reperfusion. Methods: Rabbits were subjected to myocardial ischemia/reperfusion injury and received either intravenous minocycline (n = 8) or saline (n = 8) upon reperfusion. Cardiac cell death was assessed by in vivo micro-SPECT/CT after injection of Indium-111-labeled 4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid (¹¹¹In-GSAO). Thereafter, hearts were explanted for ex vivo imaging, γ-counting, and histopathological characterization. Results: Myocardial damage was visualized by micro-SPECT/CT imaging. Quantitative GSAO uptake (expressed as percent injected dose per gram, %ID/g) in the area at risk was lower in minocycline-treated animals than that in saline-treated control animals (0.32 ± 0.13% vs 0.48 ± 0.15%, P = 0.04). TUNEL staining confirmed the reduction of cell death in minocycline-treated animals. Conclusions: This study demonstrates cardioprotection by minocycline in a clinically translatable protocol.

Original language	English
Pages (from-to)	94-100
Number of pages	7
Journal	Journal of Nuclear Cardiology
Volume	25
Issue number	1
DOIs	https://doi.org/10.1007/s12350-017-1031-8
State	Published - 1 Feb 2018

Keywords

Molecular imaging
SPECT
acute coronary syndromes
apoptosis imaging
necrosis

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10.1007/s12350-017-1031-8

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Yamaki, T., de Haas, H. J., Tahara, N., Petrov, A., Mohar, D., Haider, N., Zhou, J., Tahara, A., Takeishi, Y., Boersma, H. H., Scarabelli, T., Kini, A., Strauss, H. W., & Narula, J. (2018). Cardioprotection by minocycline in a rabbit model of ischemia/reperfusion injury: Detection of cell death by in vivo ¹¹¹In-GSAO SPECT. Journal of Nuclear Cardiology, 25(1), 94-100. https://doi.org/10.1007/s12350-017-1031-8

@article{66472e52bc5e4141be4a6986c334fa4f,

title = "Cardioprotection by minocycline in a rabbit model of ischemia/reperfusion injury: Detection of cell death by in vivo 111In-GSAO SPECT",

abstract = "Background: Preclinical studies indicate that minocycline protects against myocardial ischemia/reperfusion injury. In these studies, minocycline was administered before ischemia, which can rarely occur in clinical practice. The current study aimed to evaluate cardioprotection by minocycline treatment upon reperfusion. Methods: Rabbits were subjected to myocardial ischemia/reperfusion injury and received either intravenous minocycline (n = 8) or saline (n = 8) upon reperfusion. Cardiac cell death was assessed by in vivo micro-SPECT/CT after injection of Indium-111-labeled 4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid (111In-GSAO). Thereafter, hearts were explanted for ex vivo imaging, γ-counting, and histopathological characterization. Results: Myocardial damage was visualized by micro-SPECT/CT imaging. Quantitative GSAO uptake (expressed as percent injected dose per gram, %ID/g) in the area at risk was lower in minocycline-treated animals than that in saline-treated control animals (0.32 ± 0.13% vs 0.48 ± 0.15%, P = 0.04). TUNEL staining confirmed the reduction of cell death in minocycline-treated animals. Conclusions: This study demonstrates cardioprotection by minocycline in a clinically translatable protocol.",

keywords = "Molecular imaging, SPECT, acute coronary syndromes, apoptosis imaging, necrosis",

author = "Takayoshi Yamaki and {de Haas}, {Hans J.} and Nobuhiro Tahara and Artiom Petrov and Dilbahar Mohar and Nezam Haider and Jun Zhou and Atsuko Tahara and Yasuchika Takeishi and Boersma, {Hendrikus H.} and Tiziano Scarabelli and Annapoorna Kini and Strauss, {H. William} and Jagat Narula",

note = "Publisher Copyright: {\textcopyright} 2017, American Society of Nuclear Cardiology.",

year = "2018",

month = feb,

day = "1",

doi = "10.1007/s12350-017-1031-8",

language = "English",

volume = "25",

pages = "94--100",

journal = "Journal of Nuclear Cardiology",

issn = "1071-3581",

publisher = "American Society of Nuclear Cardiology",

number = "1",

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Yamaki, T, de Haas, HJ, Tahara, N, Petrov, A, Mohar, D, Haider, N, Zhou, J, Tahara, A, Takeishi, Y, Boersma, HH, Scarabelli, T, Kini, A, Strauss, HW & Narula, J 2018, 'Cardioprotection by minocycline in a rabbit model of ischemia/reperfusion injury: Detection of cell death by in vivo ¹¹¹In-GSAO SPECT', Journal of Nuclear Cardiology, vol. 25, no. 1, pp. 94-100. https://doi.org/10.1007/s12350-017-1031-8

TY - JOUR

T1 - Cardioprotection by minocycline in a rabbit model of ischemia/reperfusion injury

T2 - Detection of cell death by in vivo 111In-GSAO SPECT

AU - Yamaki, Takayoshi

AU - de Haas, Hans J.

AU - Tahara, Nobuhiro

AU - Petrov, Artiom

AU - Mohar, Dilbahar

AU - Haider, Nezam

AU - Zhou, Jun

AU - Tahara, Atsuko

AU - Takeishi, Yasuchika

AU - Boersma, Hendrikus H.

AU - Scarabelli, Tiziano

AU - Kini, Annapoorna

AU - Strauss, H. William

AU - Narula, Jagat

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Background: Preclinical studies indicate that minocycline protects against myocardial ischemia/reperfusion injury. In these studies, minocycline was administered before ischemia, which can rarely occur in clinical practice. The current study aimed to evaluate cardioprotection by minocycline treatment upon reperfusion. Methods: Rabbits were subjected to myocardial ischemia/reperfusion injury and received either intravenous minocycline (n = 8) or saline (n = 8) upon reperfusion. Cardiac cell death was assessed by in vivo micro-SPECT/CT after injection of Indium-111-labeled 4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid (111In-GSAO). Thereafter, hearts were explanted for ex vivo imaging, γ-counting, and histopathological characterization. Results: Myocardial damage was visualized by micro-SPECT/CT imaging. Quantitative GSAO uptake (expressed as percent injected dose per gram, %ID/g) in the area at risk was lower in minocycline-treated animals than that in saline-treated control animals (0.32 ± 0.13% vs 0.48 ± 0.15%, P = 0.04). TUNEL staining confirmed the reduction of cell death in minocycline-treated animals. Conclusions: This study demonstrates cardioprotection by minocycline in a clinically translatable protocol.

AB - Background: Preclinical studies indicate that minocycline protects against myocardial ischemia/reperfusion injury. In these studies, minocycline was administered before ischemia, which can rarely occur in clinical practice. The current study aimed to evaluate cardioprotection by minocycline treatment upon reperfusion. Methods: Rabbits were subjected to myocardial ischemia/reperfusion injury and received either intravenous minocycline (n = 8) or saline (n = 8) upon reperfusion. Cardiac cell death was assessed by in vivo micro-SPECT/CT after injection of Indium-111-labeled 4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid (111In-GSAO). Thereafter, hearts were explanted for ex vivo imaging, γ-counting, and histopathological characterization. Results: Myocardial damage was visualized by micro-SPECT/CT imaging. Quantitative GSAO uptake (expressed as percent injected dose per gram, %ID/g) in the area at risk was lower in minocycline-treated animals than that in saline-treated control animals (0.32 ± 0.13% vs 0.48 ± 0.15%, P = 0.04). TUNEL staining confirmed the reduction of cell death in minocycline-treated animals. Conclusions: This study demonstrates cardioprotection by minocycline in a clinically translatable protocol.

KW - Molecular imaging

KW - SPECT

KW - acute coronary syndromes

KW - apoptosis imaging

KW - necrosis

UR - http://www.scopus.com/inward/record.url?scp=85028301905&partnerID=8YFLogxK

U2 - 10.1007/s12350-017-1031-8

DO - 10.1007/s12350-017-1031-8

M3 - Article

C2 - 28840574

AN - SCOPUS:85028301905

SN - 1071-3581

VL - 25

SP - 94

EP - 100

JO - Journal of Nuclear Cardiology

JF - Journal of Nuclear Cardiology

IS - 1