Cardiac Tissue Engineering: Inclusion of Non-cardiomyocytes for Enhanced Features

Sadek Munawar; Irene C. Turnbull

doi:10.3389/fcell.2021.653127

Cardiac Tissue Engineering: Inclusion of Non-cardiomyocytes for Enhanced Features

Sadek Munawar, Irene C. Turnbull

Research output: Contribution to journal › Review article › peer-review

5 Scopus citations

Abstract

Engineered cardiac tissues (ECTs) are 3D physiological models of the heart that are created and studied for their potential role in developing therapies of cardiovascular diseases and testing cardio toxicity of drugs. Recreating the microenvironment of the native myocardium in vitro mainly involves the use of cardiomyocytes. However, ECTs with only cardiomyocytes (CM-only) often perform poorly and are less similar to the native myocardium compared to ECTs constructed from co-culture of cardiomyocytes and nonmyocytes. One important goal of co-culture tissues is to mimic the native heart’s cellular composition, which can result in better tissue function and maturity. In this review, we investigate the role of nonmyocytes in ECTs and discuss the mechanisms behind the contributions of nonmyocytes in enhancement of ECT features.

Original language	English
Article number	653127
Journal	Frontiers in Cell and Developmental Biology
Volume	9
DOIs	https://doi.org/10.3389/fcell.2021.653127
State	Published - 25 May 2021

Keywords

3D physiological models
cardiomyocytes
cell-cell interactions
endothelial cells
engineered cardiac tissues
fibroblasts
human induced pluripotent stem cell-derived cardiomyocytes
nonmyocytes

Access to Document

10.3389/fcell.2021.653127

Cite this

@article{9930a7379c95432c93cd3abd531e09ce,

title = "Cardiac Tissue Engineering: Inclusion of Non-cardiomyocytes for Enhanced Features",

abstract = "Engineered cardiac tissues (ECTs) are 3D physiological models of the heart that are created and studied for their potential role in developing therapies of cardiovascular diseases and testing cardio toxicity of drugs. Recreating the microenvironment of the native myocardium in vitro mainly involves the use of cardiomyocytes. However, ECTs with only cardiomyocytes (CM-only) often perform poorly and are less similar to the native myocardium compared to ECTs constructed from co-culture of cardiomyocytes and nonmyocytes. One important goal of co-culture tissues is to mimic the native heart{\textquoteright}s cellular composition, which can result in better tissue function and maturity. In this review, we investigate the role of nonmyocytes in ECTs and discuss the mechanisms behind the contributions of nonmyocytes in enhancement of ECT features.",

keywords = "3D physiological models, cardiomyocytes, cell-cell interactions, endothelial cells, engineered cardiac tissues, fibroblasts, human induced pluripotent stem cell-derived cardiomyocytes, nonmyocytes",

author = "Sadek Munawar and Turnbull, {Irene C.}",

note = "Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Munawar and Turnbull.",

year = "2021",

month = may,

day = "25",

doi = "10.3389/fcell.2021.653127",

language = "English",

volume = "9",

journal = "Frontiers in Cell and Developmental Biology",

issn = "2296-634X",

publisher = "Frontiers Media SA",

}

TY - JOUR

T1 - Cardiac Tissue Engineering

T2 - Inclusion of Non-cardiomyocytes for Enhanced Features

AU - Munawar, Sadek

AU - Turnbull, Irene C.

PY - 2021/5/25

Y1 - 2021/5/25

N2 - Engineered cardiac tissues (ECTs) are 3D physiological models of the heart that are created and studied for their potential role in developing therapies of cardiovascular diseases and testing cardio toxicity of drugs. Recreating the microenvironment of the native myocardium in vitro mainly involves the use of cardiomyocytes. However, ECTs with only cardiomyocytes (CM-only) often perform poorly and are less similar to the native myocardium compared to ECTs constructed from co-culture of cardiomyocytes and nonmyocytes. One important goal of co-culture tissues is to mimic the native heart’s cellular composition, which can result in better tissue function and maturity. In this review, we investigate the role of nonmyocytes in ECTs and discuss the mechanisms behind the contributions of nonmyocytes in enhancement of ECT features.

AB - Engineered cardiac tissues (ECTs) are 3D physiological models of the heart that are created and studied for their potential role in developing therapies of cardiovascular diseases and testing cardio toxicity of drugs. Recreating the microenvironment of the native myocardium in vitro mainly involves the use of cardiomyocytes. However, ECTs with only cardiomyocytes (CM-only) often perform poorly and are less similar to the native myocardium compared to ECTs constructed from co-culture of cardiomyocytes and nonmyocytes. One important goal of co-culture tissues is to mimic the native heart’s cellular composition, which can result in better tissue function and maturity. In this review, we investigate the role of nonmyocytes in ECTs and discuss the mechanisms behind the contributions of nonmyocytes in enhancement of ECT features.

KW - 3D physiological models

KW - cardiomyocytes

KW - cell-cell interactions

KW - endothelial cells

KW - engineered cardiac tissues

KW - fibroblasts

KW - human induced pluripotent stem cell-derived cardiomyocytes

KW - nonmyocytes

UR - http://www.scopus.com/inward/record.url?scp=85107410296&partnerID=8YFLogxK

U2 - 10.3389/fcell.2021.653127

DO - 10.3389/fcell.2021.653127

M3 - Review article

AN - SCOPUS:85107410296

SN - 2296-634X

VL - 9

JO - Frontiers in Cell and Developmental Biology

JF - Frontiers in Cell and Developmental Biology

M1 - 653127

ER -

Cardiac Tissue Engineering: Inclusion of Non-cardiomyocytes for Enhanced Features

Abstract

Keywords

Access to Document

Fingerprint

Cite this