@inbook{33c3038c9d764c9e9bf54b7bbcac8d91,
title = "Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques",
abstract = "Percutaneous antegrade coronary injection is among the least invasive cardiac selective gene delivery methods. However, the transduction efficiency of a simple bolus antegrade injection is quite low. In order to improve transduction efficiency in antegrade intracoronary delivery, several additional approaches have been proposed. In this chapter, we will describe the important elements associated with intracoronary delivery methods and present protocols for three different catheter-based antegrade gene delivery techniques in a preclinical large animal model. This is the second edition of this chapter, and it includes modifications we have made over the past several years that further enhance transduction efficacy.",
keywords = "Balloon occlusion, Cardiovascular diseases, Gene delivery, Gene therapy, Homogenous distribution, Impella, Intracoronary, Minimally invasive, Vectors",
author = "Mavropoulos, \{Spyros A.\} and Yamada, \{Kelly P.\} and Tomoki Sakata and Kiyotake Ishikawa",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2707-5\_11",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "147--158",
booktitle = "Methods in Molecular Biology",
}