Cardiac allograft rejection in the absence of macrophage migration inhibitory factor

Yilmaz Demir, Yifa Chen, Christine Metz, Harald Renz, Peter S. Heeger

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Background. Macrophage migration inhibitory factor (MIF) is a secreted proinflammatory lymphokine essential for elicitation of delayed-type hypersensitivity (DTH) reactions in vivo. We tested whether MIF blockade-absence affected acute or chronic murine cardiac allograft rejection. Methods. Wild-type (WT) C57BL/6 (B6) mice underwent transplantation with BALB/c hearts with or without blocking anti-MIF antibody, and MIF knockout (KO) B6 mice underwent transplantation with MIF KO BALB/c hearts. Chronic immune injury was induced in WT and KO recipients using donor-specific transfusion and anti-CD40L antibody. Results. Unexpectedly, the blockade or genetic absence of MIF did not prolong graft survival even if recipient T-cell cytotoxicity was additionally impaired. The histologic manifestations of acute and chronic immune injury to the allograft were similar between groups. Conclusions. MIF is not required for acute or chronic allograft rejection in mice. The findings raise questions about the role of DTH as an important mediator of cardiac allograft injury.

Original languageEnglish
Pages (from-to)244-247
Number of pages4
Issue number1
StatePublished - 15 Jul 2003
Externally publishedYes


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