Capturing the in vivo molecular signature of the podocyte

Peter Y. Chuang; Jia Fu; John C. He

doi:10.1038/ki.2013.65

Capturing the in vivo molecular signature of the podocyte

Peter Y. Chuang, Jia Fu, John C. He

Research output: Contribution to journal › Comment/debate

1 Scopus citations

Abstract

The podocyte has an essential role in glomerular function. When cultured ex vivo, podocytes do not recapitulate their in vivo phenotype. Several technical barriers have prevented isolation of podocytes from animals with sufficient yield and purity to allow genome-wide assessment of their molecular fingerprint. Boerries et al. overcame some of these barriers and characterized the transcriptome and proteome of freshly isolated podocytes. These data sets and isolation protocol are valuable resources for the podocyte research community.

Original language	English
Pages (from-to)	986-988
Number of pages	3
Journal	Kidney International
Volume	83
Issue number	6
DOIs	https://doi.org/10.1038/ki.2013.65
State	Published - Jun 2013

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10.1038/ki.2013.65

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title = "Capturing the in vivo molecular signature of the podocyte",

abstract = "The podocyte has an essential role in glomerular function. When cultured ex vivo, podocytes do not recapitulate their in vivo phenotype. Several technical barriers have prevented isolation of podocytes from animals with sufficient yield and purity to allow genome-wide assessment of their molecular fingerprint. Boerries et al. overcame some of these barriers and characterized the transcriptome and proteome of freshly isolated podocytes. These data sets and isolation protocol are valuable resources for the podocyte research community.",

author = "Chuang, {Peter Y.} and Jia Fu and He, {John C.}",

note = "Funding Information: P.Y.C. is supported by NIH 5K08-DK082760. J.F. is supported by Chinese 973 fund 2012CB517601. J.C.H. is supported by NIH 1R01-DK088541, P01-DK56492, and a Veterans Affairs Merit Award.",

year = "2013",

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volume = "83",

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journal = "Kidney International",

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T1 - Capturing the in vivo molecular signature of the podocyte

AU - Chuang, Peter Y.

AU - Fu, Jia

AU - He, John C.

N1 - Funding Information: P.Y.C. is supported by NIH 5K08-DK082760. J.F. is supported by Chinese 973 fund 2012CB517601. J.C.H. is supported by NIH 1R01-DK088541, P01-DK56492, and a Veterans Affairs Merit Award.

PY - 2013/6

Y1 - 2013/6

N2 - The podocyte has an essential role in glomerular function. When cultured ex vivo, podocytes do not recapitulate their in vivo phenotype. Several technical barriers have prevented isolation of podocytes from animals with sufficient yield and purity to allow genome-wide assessment of their molecular fingerprint. Boerries et al. overcame some of these barriers and characterized the transcriptome and proteome of freshly isolated podocytes. These data sets and isolation protocol are valuable resources for the podocyte research community.

AB - The podocyte has an essential role in glomerular function. When cultured ex vivo, podocytes do not recapitulate their in vivo phenotype. Several technical barriers have prevented isolation of podocytes from animals with sufficient yield and purity to allow genome-wide assessment of their molecular fingerprint. Boerries et al. overcame some of these barriers and characterized the transcriptome and proteome of freshly isolated podocytes. These data sets and isolation protocol are valuable resources for the podocyte research community.

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DO - 10.1038/ki.2013.65

M3 - Comment/debate

AN - SCOPUS:84882281932

SN - 0085-2538

VL - 83

SP - 986

EP - 988

JO - Kidney International

JF - Kidney International

IS - 6

ER -

Capturing the in vivo molecular signature of the podocyte

Abstract

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