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Capillary electrophoresis for the analysis of glycosaminoglycan-derived disaccharides

  • Yuqing Chang
  • , Bo Yang
  • , Amanda Weyers
  • , Robert J. Linhardt

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

7 Scopus citations

Abstract

Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination, which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapter is a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.

Original languageEnglish
Title of host publicationCapillary Electrophoresis of Biomolecules
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages67-77
Number of pages11
ISBN (Print)9781627032957
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume984
ISSN (Print)1064-3745

Keywords

  • 2-Aminoacridone
  • Capillary electrophoresis
  • Disaccharide
  • Glycosaminoglycan
  • Laserinduced fluorescence

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