Cancer-testis antigens are commonly expressed in multiple myeloma and induce systemic immunity following allogeneic stem cell transplantation

Djordje Atanackovic, Julia Arfsten, Yanran Cao, Sacha Gnjatic, Frank Schnieders, Katrin Bartels, Georgia Schilling, Christiane Faltz, Christine Wolschke, Judith Dierlamm, Gerd Ritter, Thomas Eiermann, Dieter Kurt Hossfeld, Axel R. Zander, Achim A. Jungbluth, Lloyd J. Old, Carsten Bokemeyer, Nicolaus Kröger

Research output: Contribution to journalArticlepeer-review

144 Scopus citations

Abstract

Immunotherapies using cancer-testis (CT) antigens as targets represent a potentially useful treatment in patients with multiple myeloma (MM) who commonly show recurrent disease following chemotherapy. We analyzed the expression of 11 CT antigens in bone marrow samples from patients with MM (n = 55) and healthy donors (n = 32) using reverse transcriptase-polymerase chain reaction (RT-PCR). CT antigens were frequently expressed in MM with 56% (MAGEC2), 55% (MAGEA3), 35% (SSX1), 20% (SSX4, SSX5), 16% (SSX2), 15% (BAGE), 7% (NYESO-1), and 6% (ADAM2, LIPI) expressing the given antigen. Importantly, CT antigens were not expressed in healthy bone marrow. Analyzing patients with MM (n = 66) for antibody responses against MAGEA3, SSX2, and NY-ESO-1, we found strong antibody responses against CT antigens preferentially in patients who had received allogeneic stem cell transplantation (alloSCT). Antibody responses against NY-ESO-1 correlated with NY-ESO-1-specific CD4+ and CD8+ T-cell responses against peptide NY-ESO-151-62 and CD4+ responses against NY-ESO-1121-140 in 1 of these patients. These allogeneic immune responses were not detectable in pretransplantation samples and in the patients' stem cell donors, indicating that CT antigens might indeed represent natural targets for graft-versus-myeloma effects. Immune responses induced by alloSCT could be boosted by active CT antigen-specific immunotherapy, which might help to achieve long-lasting remissions in patients with MM.

Original languageEnglish
Pages (from-to)1103-1112
Number of pages10
JournalBlood
Volume109
Issue number3
DOIs
StatePublished - 1 Feb 2007
Externally publishedYes

Fingerprint

Dive into the research topics of 'Cancer-testis antigens are commonly expressed in multiple myeloma and induce systemic immunity following allogeneic stem cell transplantation'. Together they form a unique fingerprint.

Cite this