Cancer-testis Antigen7 expression and immune responses following allogeneic stem cell transplantation for multiple myeloma

Eleanor M. Tyler, Achim A. Jungbluth, Sacha Gnjatic, Richard J. O'Reilly, Guenther Koehne

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Cancer-testis antigen 7 (CT7) is the most frequently and consistently expressed MAGE antigen in multiple myeloma, exhibits tissue-restricted expression, and is an independent negative prognostic factor for multiple myeloma. We sought to characterize CT7 protein expression in the bone marrow of patients with multiple myeloma undergoing allogeneic T cell-depleted hematopoietic stem cell transplantation (alloTCD-HSCT), and to examine the significance of CT7-specific cellular immune responses. We further aimed to determine CT7-derived immunogenic epitopes and their associated allelic restrictions. CT7 protein expression in neoplastic CD138 plasma cells was evaluated by immunohistochemistry in bone marrow biopsies from 10 patients. CT7 was present in 8 of 10 patients. Longitudinal analyses of the 10 patients revealed an association between CT7 expression and prognosis. Longitudinal monitoring of CT7-specific T cells revealed an association between increased frequencies of CT7-specific T cells and reductions in specific myeloma markers. Epitope-specific reactivity to the nonamer FLAMLKNTV was detected by intracellular IFNγ assay in peripheral blood (PB) and bone marrow-derived T cells from HLA-A0201+ patients. Serial monitoring of PB CT7-specific T-cell frequencies in 4 HLA-A0201+ patients by HLA-A0201-CT7(1087-1095) tetramer staining revealed an association with disease course. Phenotypic analyses revealed bone marrow enrichment for central memory CT7-specific T cells, while effector memory cells dominated the PB. Together, these findings support the development of immunotherapeutic strategies that aim to enhance CT7-directed immune responses for the treatment of multiple myeloma.

Original languageEnglish
Pages (from-to)547-558
Number of pages12
JournalCancer Immunology Research
Volume2
Issue number6
DOIs
StatePublished - Jun 2014

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