CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible Spectrum

Peyton Shieh, Vivian T. Dien, Brendan J. Beahm, Joseph M. Castellano, Tony Wyss-Coray, Carolyn R. Bertozzi

Research output: Contribution to journalArticlepeer-review

126 Scopus citations

Abstract

Fluorescent bioorthogonal smart probes across the visible spectrum will enable sensitive visualization of metabolically labeled molecules in biological systems. Here we present a unified design, based on the principle of photoinduced electron transfer, to access a panel of highly fluorogenic azide probes that are activated by conversion to the corresponding triazoles via click chemistry. Termed the CalFluors, these probes possess emission maxima that range from green to far red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. We used the CalFluor probes to image various alkyne-labeled biomolecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices.

Original languageEnglish
Pages (from-to)7145-7151
Number of pages7
JournalJournal of the American Chemical Society
Volume137
Issue number22
DOIs
StatePublished - 10 Jun 2015
Externally publishedYes

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