Calcium Uncaging with Visible Light

Hitesh K. Agarwal; Radoslav Janicek; San Hui Chi; Joseph W. Perry; Ernst Niggli; Graham C.R. Ellis-Davies

doi:10.1021/jacs.5b11606

Calcium Uncaging with Visible Light

Hitesh K. Agarwal
, Radoslav Janicek
, San Hui Chi
, Joseph W. Perry
, Ernst Niggli
, Graham C.R. Ellis-Davies

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

We have designed a nitroaromatic photochemical protecting group that absorbs visible light in the violet-blue range. The chromophore is a dinitro derivative of bisstyrylthiophene (or BIST) that absorbs light very effectively (ε₄₄₀ = 66,000 M^-1 cm^-1 and two-photon cross section of 350 GM at 775 nm). We developed a "caged calcium" molecule by conjugation of BIST to a Ca²⁺ chelator that upon laser flash photolysis rapidly releases Ca²⁺ in <0.2 ms. Using the patch-clamp method the optical probe, loaded with Ca²⁺, was delivered into acutely isolated mouse cardiac myocytes, where either one- and two-photon uncaging of Ca²⁺ induced highly local or cell-wide physiological Ca²⁺ signaling events.

Original language	English
Pages (from-to)	3687-3693
Number of pages	7
Journal	Journal of the American Chemical Society
Volume	138
Issue number	11
DOIs	https://doi.org/10.1021/jacs.5b11606
State	Published - 23 Mar 2016

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abstract = "We have designed a nitroaromatic photochemical protecting group that absorbs visible light in the violet-blue range. The chromophore is a dinitro derivative of bisstyrylthiophene (or BIST) that absorbs light very effectively (ε440 = 66,000 M-1 cm-1 and two-photon cross section of 350 GM at 775 nm). We developed a {"}caged calcium{"} molecule by conjugation of BIST to a Ca2+ chelator that upon laser flash photolysis rapidly releases Ca2+ in <0.2 ms. Using the patch-clamp method the optical probe, loaded with Ca2+, was delivered into acutely isolated mouse cardiac myocytes, where either one- and two-photon uncaging of Ca2+ induced highly local or cell-wide physiological Ca2+ signaling events.",

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AU - Agarwal, Hitesh K.

AU - Janicek, Radoslav

AU - Chi, San Hui

AU - Perry, Joseph W.

AU - Niggli, Ernst

AU - Ellis-Davies, Graham C.R.

PY - 2016/3/23

Y1 - 2016/3/23

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AB - We have designed a nitroaromatic photochemical protecting group that absorbs visible light in the violet-blue range. The chromophore is a dinitro derivative of bisstyrylthiophene (or BIST) that absorbs light very effectively (ε440 = 66,000 M-1 cm-1 and two-photon cross section of 350 GM at 775 nm). We developed a "caged calcium" molecule by conjugation of BIST to a Ca2+ chelator that upon laser flash photolysis rapidly releases Ca2+ in <0.2 ms. Using the patch-clamp method the optical probe, loaded with Ca2+, was delivered into acutely isolated mouse cardiac myocytes, where either one- and two-photon uncaging of Ca2+ induced highly local or cell-wide physiological Ca2+ signaling events.

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 11

ER -

Calcium Uncaging with Visible Light

Abstract

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