Calcium buffering capacity of neuronal cell cytosol measured by flash photolysis of calcium buffer NP-EGTA

Amanda Fleet; Graham Ellis-Davies; Stephen Bolsover

doi:10.1006/bbrc.1998.9377

Calcium buffering capacity of neuronal cell cytosol measured by flash photolysis of calcium buffer NP-EGTA

Amanda Fleet, Graham Ellis-Davies, Stephen Bolsover

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

N1E-115 mouse neuroblastoma cells were injected with a calcium buffer/indicator solution to allow both ratiometric measurement of free calcium concentration and the release of calcium ions upon UV flash. The solution contained sulforhodamine, a marker dye used to estimate the volume injected; fluo-3, a calcium indicator, and NP-EGTA, a high affinity calcium-selective buffer that is converted by UV flash to products with negligible calcium affinity. The calcium increase recorded upon UV irradiation (Δ[Ca²⁺](i)) was small for small injection volumes, increased with larger injection volumes, but approached a plateau at the largest injection volumes. From this relation we estimate the buffering capacity of the cytosol as 1700 ions bound per ion free.

Original language	English
Pages (from-to)	786-790
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	250
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1998.9377
State	Published - 29 Sep 1998
Externally published	Yes

Keywords

Calcium buffering
Calcium homeostasis
Flash photolysis
Nerve cell

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10.1006/bbrc.1998.9377

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title = "Calcium buffering capacity of neuronal cell cytosol measured by flash photolysis of calcium buffer NP-EGTA",

abstract = "N1E-115 mouse neuroblastoma cells were injected with a calcium buffer/indicator solution to allow both ratiometric measurement of free calcium concentration and the release of calcium ions upon UV flash. The solution contained sulforhodamine, a marker dye used to estimate the volume injected; fluo-3, a calcium indicator, and NP-EGTA, a high affinity calcium-selective buffer that is converted by UV flash to products with negligible calcium affinity. The calcium increase recorded upon UV irradiation (Δ[Ca2+](i)) was small for small injection volumes, increased with larger injection volumes, but approached a plateau at the largest injection volumes. From this relation we estimate the buffering capacity of the cytosol as 1700 ions bound per ion free.",

keywords = "Calcium buffering, Calcium homeostasis, Flash photolysis, Nerve cell",

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AU - Bolsover, Stephen

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Calcium buffering capacity of neuronal cell cytosol measured by flash photolysis of calcium buffer NP-EGTA

Abstract

Keywords

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