Calcium binding properties of rabbit liver protein disulfide isomerase

Djamel Lebeche; Hector A. Lucero; Benjamin Kaminer

doi:10.1006/bbrc.1994.1964

Calcium binding properties of rabbit liver protein disulfide isomerase

Djamel Lebeche
, Hector A. Lucero
, Benjamin Kaminer

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

We have characterized the Ca²⁺ binding properties of protein disulfide isomerase (PDI). It binds 19 mol Ca²⁺/mol protein with low affinity, which is reduced by increasing the ionic strength. Ca²⁺ induced conformational changes detected by UV difference spectroscopy. These Ca²⁺ binding properties resemble those of a sea urchin egg 58 kDa protein.

Original language	English
Pages (from-to)	556-561
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	202
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1994.1964
State	Published - 15 Jul 1994
Externally published	Yes

Access to Document

10.1006/bbrc.1994.1964

Cite this

@article{f3be75e9561241f79441d629b6286284,

title = "Calcium binding properties of rabbit liver protein disulfide isomerase",

abstract = "We have characterized the Ca2+ binding properties of protein disulfide isomerase (PDI). It binds 19 mol Ca2+/mol protein with low affinity, which is reduced by increasing the ionic strength. Ca2+ induced conformational changes detected by UV difference spectroscopy. These Ca2+ binding properties resemble those of a sea urchin egg 58 kDa protein.",

author = "Djamel Lebeche and Lucero, \{Hector A.\} and Benjamin Kaminer",

year = "1994",

month = jul,

day = "15",

doi = "10.1006/bbrc.1994.1964",

language = "English",

volume = "202",

pages = "556--561",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Calcium binding properties of rabbit liver protein disulfide isomerase

AU - Lebeche, Djamel

AU - Lucero, Hector A.

AU - Kaminer, Benjamin

PY - 1994/7/15

Y1 - 1994/7/15

N2 - We have characterized the Ca2+ binding properties of protein disulfide isomerase (PDI). It binds 19 mol Ca2+/mol protein with low affinity, which is reduced by increasing the ionic strength. Ca2+ induced conformational changes detected by UV difference spectroscopy. These Ca2+ binding properties resemble those of a sea urchin egg 58 kDa protein.

AB - We have characterized the Ca2+ binding properties of protein disulfide isomerase (PDI). It binds 19 mol Ca2+/mol protein with low affinity, which is reduced by increasing the ionic strength. Ca2+ induced conformational changes detected by UV difference spectroscopy. These Ca2+ binding properties resemble those of a sea urchin egg 58 kDa protein.

UR - https://www.scopus.com/pages/publications/0028093077

U2 - 10.1006/bbrc.1994.1964

DO - 10.1006/bbrc.1994.1964

M3 - Article

AN - SCOPUS:0028093077

SN - 0006-291X

VL - 202

SP - 556

EP - 561

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Calcium binding properties of rabbit liver protein disulfide isomerase

Abstract

Access to Document

Fingerprint

Cite this