Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase

Nicholas K. Tonks; Philip Cohen

doi:10.1016/0167-4838(83)90140-1

Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase

Nicholas K. Tonks
, Philip Cohen

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

A Ca²⁺-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca²⁺, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca²⁺-dependent protein phosphatase, and that interaction of Ca²⁺ with the B-subunit is essential for substrate binding.

Original language	English
Pages (from-to)	191-193
Number of pages	3
Journal	Biochimica et Biophysica Acta - Proteins and Proteomics
Volume	747
Issue number	1-2
DOIs	https://doi.org/10.1016/0167-4838(83)90140-1
State	Published - 14 Sep 1983
Externally published	Yes

Keywords

Affinity chromatography
Ca-dependent protein phosphatase
Calcineurin
Calmodulin binding protein

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10.1016/0167-4838(83)90140-1

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title = "Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase",

abstract = "A Ca2+-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca2+-dependent protein phosphatase, and that interaction of Ca2+ with the B-subunit is essential for substrate binding.",

keywords = "Affinity chromatography, Ca-dependent protein phosphatase, Calcineurin, Calmodulin binding protein",

author = "Tonks, \{Nicholas K.\} and Philip Cohen",

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T1 - Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase

AU - Tonks, Nicholas K.

AU - Cohen, Philip

PY - 1983/9/14

Y1 - 1983/9/14

N2 - A Ca2+-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca2+-dependent protein phosphatase, and that interaction of Ca2+ with the B-subunit is essential for substrate binding.

AB - A Ca2+-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca2+-dependent protein phosphatase, and that interaction of Ca2+ with the B-subunit is essential for substrate binding.

KW - Affinity chromatography

KW - Ca-dependent protein phosphatase

KW - Calcineurin

KW - Calmodulin binding protein

UR - https://www.scopus.com/pages/publications/0021030632

U2 - 10.1016/0167-4838(83)90140-1

DO - 10.1016/0167-4838(83)90140-1

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AN - SCOPUS:0021030632

SN - 0167-4838

VL - 747

SP - 191

EP - 193

JO - Biochimica et Biophysica Acta - Proteins and Proteomics

JF - Biochimica et Biophysica Acta - Proteins and Proteomics

IS - 1-2

ER -

Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase

Abstract

Keywords

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