Calbindin controls release probability in ventral tegmental area dopamine neurons

Ping Yue Pan; Timothy A. Ryan

doi:10.1038/nn.3099

Calbindin controls release probability in ventral tegmental area dopamine neurons

Ping Yue Pan
, Timothy A. Ryan

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Relatively little is known about the molecular control of midbrain dopamine release. Using high-fidelity imaging of pHluorin-tagged vesicular monoamine transporter 2 in dopamine neurons, we found that exocytosis was more loosely coupled to calcium entry than in fast synapses. In ventral tegmental area neurons, this allows exocytosis to be efficiently controlled by a native fast calcium buffer, calbindin-D28k, maintaining a lower vesicular release probability compared with substantia nigra neurons.

Original language	English
Pages (from-to)	813-815
Number of pages	3
Journal	Nature Neuroscience
Volume	15
Issue number	6
DOIs	https://doi.org/10.1038/nn.3099
State	Published - Jun 2012
Externally published	Yes

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title = "Calbindin controls release probability in ventral tegmental area dopamine neurons",

abstract = "Relatively little is known about the molecular control of midbrain dopamine release. Using high-fidelity imaging of pHluorin-tagged vesicular monoamine transporter 2 in dopamine neurons, we found that exocytosis was more loosely coupled to calcium entry than in fast synapses. In ventral tegmental area neurons, this allows exocytosis to be efficiently controlled by a native fast calcium buffer, calbindin-D28k, maintaining a lower vesicular release probability compared with substantia nigra neurons.",

author = "Pan, \{Ping Yue\} and Ryan, \{Timothy A.\}",

note = "Funding Information: We wish to thank R. Edwards (University of California San Francisco) for generously providing VMAT-pHluorin, A. Lee (University of Iowa) for providing pcDNA3.1–calbindin-D28k, A. Miyawaki (RIKEN) for providing CY3.60 and members of the Ryan laboratory for helpful discussions. This work was supported by funds from the National Institute on Drug Abuse (DA0101540) and the National Institute of Mental Health (MH085783).",

year = "2012",

month = jun,

doi = "10.1038/nn.3099",

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AU - Ryan, Timothy A.

N1 - Funding Information: We wish to thank R. Edwards (University of California San Francisco) for generously providing VMAT-pHluorin, A. Lee (University of Iowa) for providing pcDNA3.1–calbindin-D28k, A. Miyawaki (RIKEN) for providing CY3.60 and members of the Ryan laboratory for helpful discussions. This work was supported by funds from the National Institute on Drug Abuse (DA0101540) and the National Institute of Mental Health (MH085783).

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N2 - Relatively little is known about the molecular control of midbrain dopamine release. Using high-fidelity imaging of pHluorin-tagged vesicular monoamine transporter 2 in dopamine neurons, we found that exocytosis was more loosely coupled to calcium entry than in fast synapses. In ventral tegmental area neurons, this allows exocytosis to be efficiently controlled by a native fast calcium buffer, calbindin-D28k, maintaining a lower vesicular release probability compared with substantia nigra neurons.

AB - Relatively little is known about the molecular control of midbrain dopamine release. Using high-fidelity imaging of pHluorin-tagged vesicular monoamine transporter 2 in dopamine neurons, we found that exocytosis was more loosely coupled to calcium entry than in fast synapses. In ventral tegmental area neurons, this allows exocytosis to be efficiently controlled by a native fast calcium buffer, calbindin-D28k, maintaining a lower vesicular release probability compared with substantia nigra neurons.

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JO - Nature Neuroscience

JF - Nature Neuroscience

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ER -

Calbindin controls release probability in ventral tegmental area dopamine neurons

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