Bromine analysis in 5-bromouracil-labeled DNA by X-ray fluorescence

L. Zeitz, R. Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The degree of replacement of thymine by 5-bromouracil in the deoxyribonucleic acid (DNA) of the thy mine-deficient bacterium Escherichia coli B 15 T- has been determined by a nondispersive x-ray fluorescence method. A measure of the fraction by weight of bromine in the purified DNA is required for determining the degree of thymine replacement. This fraction is experimentally determined on 35-μg samples of DNA by x-ray spectroanalysis.

Original languageEnglish
Pages (from-to)1670-1673
Number of pages4
JournalScience
Volume142
Issue number3600
DOIs
StatePublished - 1963
Externally publishedYes

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