BrdU-Hoechst flow cytometry: A unique tool for quantitative cell cycle analysis

P. S. Rabinovitch; M. Kubbies; Y. C. Chen; D. Schindler; H. Hoehn

doi:10.1016/0014-4827(88)90302-3

BrdU-Hoechst flow cytometry: A unique tool for quantitative cell cycle analysis

P. S. Rabinovitch
, M. Kubbies
, Y. C. Chen
, D. Schindler
, H. Hoehn

Research output: Contribution to journal › Article › peer-review

140 Scopus citations

Abstract

Unlike other techniques, flow cytometric analysis of BrdU-quenched 33258 Hoechst fluorescence may be used to measure cell activation and the G₁, S, and G₂ M compartment distributions in each of three successive cell cycles after growth stimulation of human peripheral blood lymphocytes. Cell cycle kinetic curves can be constructed from the BrdU-Hoechst flow data which allow the simultaneous assessment of growth fraction, lagtime, compartment exit rate, compartment duration, and compartment arrest. Applications of this new versatile technique include the evaluation of drug and growth factor effets, cell aging, and diagnosis in medicine and immunology.

Original language	English
Pages (from-to)	309-318
Number of pages	10
Journal	Experimental Cell Research
Volume	174
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(88)90302-3
State	Published - Feb 1988
Externally published	Yes

Access to Document

10.1016/0014-4827(88)90302-3

Cite this

@article{77f3dd49faf742fea3463d57458aee87,

title = "BrdU-Hoechst flow cytometry: A unique tool for quantitative cell cycle analysis",

abstract = "Unlike other techniques, flow cytometric analysis of BrdU-quenched 33258 Hoechst fluorescence may be used to measure cell activation and the G1, S, and G2 M compartment distributions in each of three successive cell cycles after growth stimulation of human peripheral blood lymphocytes. Cell cycle kinetic curves can be constructed from the BrdU-Hoechst flow data which allow the simultaneous assessment of growth fraction, lagtime, compartment exit rate, compartment duration, and compartment arrest. Applications of this new versatile technique include the evaluation of drug and growth factor effets, cell aging, and diagnosis in medicine and immunology.",

author = "Rabinovitch, \{P. S.\} and M. Kubbies and Chen, \{Y. C.\} and D. Schindler and H. Hoehn",

note = "Funding Information: Grant AGO1751a nd DFG",

year = "1988",

month = feb,

doi = "10.1016/0014-4827(88)90302-3",

language = "English",

volume = "174",

pages = "309--318",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - BrdU-Hoechst flow cytometry

T2 - A unique tool for quantitative cell cycle analysis

AU - Rabinovitch, P. S.

AU - Kubbies, M.

AU - Chen, Y. C.

AU - Schindler, D.

AU - Hoehn, H.

N1 - Funding Information: Grant AGO1751a nd DFG

PY - 1988/2

Y1 - 1988/2

N2 - Unlike other techniques, flow cytometric analysis of BrdU-quenched 33258 Hoechst fluorescence may be used to measure cell activation and the G1, S, and G2 M compartment distributions in each of three successive cell cycles after growth stimulation of human peripheral blood lymphocytes. Cell cycle kinetic curves can be constructed from the BrdU-Hoechst flow data which allow the simultaneous assessment of growth fraction, lagtime, compartment exit rate, compartment duration, and compartment arrest. Applications of this new versatile technique include the evaluation of drug and growth factor effets, cell aging, and diagnosis in medicine and immunology.

AB - Unlike other techniques, flow cytometric analysis of BrdU-quenched 33258 Hoechst fluorescence may be used to measure cell activation and the G1, S, and G2 M compartment distributions in each of three successive cell cycles after growth stimulation of human peripheral blood lymphocytes. Cell cycle kinetic curves can be constructed from the BrdU-Hoechst flow data which allow the simultaneous assessment of growth fraction, lagtime, compartment exit rate, compartment duration, and compartment arrest. Applications of this new versatile technique include the evaluation of drug and growth factor effets, cell aging, and diagnosis in medicine and immunology.

UR - https://www.scopus.com/pages/publications/0023841617

U2 - 10.1016/0014-4827(88)90302-3

DO - 10.1016/0014-4827(88)90302-3

M3 - Article

C2 - 2448151

AN - SCOPUS:0023841617

SN - 0014-4827

VL - 174

SP - 309

EP - 318

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

BrdU-Hoechst flow cytometry: A unique tool for quantitative cell cycle analysis

Abstract

Access to Document

Fingerprint

Cite this