BrdU-Hoechst flow cytometry: A unique tool for quantitative cell cycle analysis

P. S. Rabinovitch, M. Kubbies, Y. C. Chen, D. Schindler, H. Hoehn

Research output: Contribution to journalArticlepeer-review

131 Scopus citations

Abstract

Unlike other techniques, flow cytometric analysis of BrdU-quenched 33258 Hoechst fluorescence may be used to measure cell activation and the G1, S, and G2 M compartment distributions in each of three successive cell cycles after growth stimulation of human peripheral blood lymphocytes. Cell cycle kinetic curves can be constructed from the BrdU-Hoechst flow data which allow the simultaneous assessment of growth fraction, lagtime, compartment exit rate, compartment duration, and compartment arrest. Applications of this new versatile technique include the evaluation of drug and growth factor effets, cell aging, and diagnosis in medicine and immunology.

Original languageEnglish
Pages (from-to)309-318
Number of pages10
JournalExperimental Cell Research
Volume174
Issue number2
DOIs
StatePublished - Feb 1988
Externally publishedYes

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