TY - JOUR
T1 - Brain regional distribution of prion protein prp27-30 in mice stereotaxically microinjected with different strains of scrapie
AU - Casaccia-Bonnefil, Patrizia
AU - Kascsak, Richard J.
AU - Fersko, Regina
AU - Callahan, Sharon
AU - Carp, Richard I.
N1 - Funding Information:
Received 7 April 1992: revised 6 August 1992. Presented in part: VIII International Congress of Virology. Berlin. August 1990. Grant support: National Institutes ofHealth (NS-21349). New York State Office of Mental Retardation and Developmental Disabilities. Fidia Research Foundation (fellowship to P.C-B.). Italy. Reprints or correspondence: Dr. Richard Kascsak, Laboratory of Immunological Neurovirology, New York State Institute for Basic Research. 1050 Forest Hill Rd.. Staten Island. NY 10314. *Presentaffiliation: State UniversityofNewYork. HealthScienceCenter at Brooklyn.
PY - 1993/1
Y1 - 1993/1
N2 - Stereotaxic inoculation was used to examine the role of scrapie agent strain, inoculum, and injection site on the brain regional distribution ofthe prion protein, PrP27-30. Neither the type of inoculum nor the injection site influenced the distribution of PrP27-30 in brains of mice. Among the parameters examined, only the strain of agent affected the pattern of distribution and the yield of PrP27-30. Although mice injected into the cerebellum had the shortest incubation period, the cerebellum gave the lowest yield of the PrP27-30 among the seven brain regions examined. The positive correlation between PrP27-30 regional distribution and lesion profile (degree of vacuolation) reinforces the role of the Prpsc protein in scrapie pathogenesis.
AB - Stereotaxic inoculation was used to examine the role of scrapie agent strain, inoculum, and injection site on the brain regional distribution ofthe prion protein, PrP27-30. Neither the type of inoculum nor the injection site influenced the distribution of PrP27-30 in brains of mice. Among the parameters examined, only the strain of agent affected the pattern of distribution and the yield of PrP27-30. Although mice injected into the cerebellum had the shortest incubation period, the cerebellum gave the lowest yield of the PrP27-30 among the seven brain regions examined. The positive correlation between PrP27-30 regional distribution and lesion profile (degree of vacuolation) reinforces the role of the Prpsc protein in scrapie pathogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0027472054&partnerID=8YFLogxK
U2 - 10.1093/infdis/167.1.7
DO - 10.1093/infdis/167.1.7
M3 - Article
C2 - 8093365
AN - SCOPUS:0027472054
SN - 0022-1899
VL - 167
SP - 7
EP - 12
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -