Boxb mediate BALB/c mice corneal inflammation through a TLR4/Myd88-dependent signaling pathway in Aspergillus fumigatus keratitis

Min Liu, Cui Li, Gui Qiu Zhao, Jing Lin, Cheng Ye Che, Qiang Xu, Qian Wang, Rui Xu, Ya Wen Niu

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

● AIM: To investigate whether high-mobility group box 1 (HMGB1) Boxb exacerbates BALB/c mice corneal immune responses and inflammatory through the Toll-like receptor 4 (TLR4)/myeloid differentiation primary response 88 (MyD88)-dependent signaling pathway in Aspergillus fumigatus (A. fumigatus) keratitis. ● METHODS: The mice corneas were pretreated with phosphate buffer saline (PBS), Boxb before A. fumigatus infection. The abdominal cavity extracted macrophages were pretreated with PBS, Boxb, TLR4 inhibitor (CLI-095), Dimethyl sulfoxide (DMSO) separately before A. fumigatus hyphae stimulation. HMGB1 was detected in normal and infected mice corneas and macrophages by real-time reverse transcriptase polymerase chain reaction (RT-PCR), the TLR4, MyD88, interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) were detected by Western blot and PCR. ● RESULTS: In BALB/c mice corneas, the expressions of TLR4, HMGB1, IL-1β, TNF-α were increased after A. fumigatus infection. While pretreatment with Boxb significantly increased the expressions of TLR4, HMGB1, MyD88, IL- 1β, TNF-α compared with PBS control after infection. In BALB/c mice abdominal cavity extracted macrophages, pretreatment with Boxb increased the expressions of TLR4, HMGB1, MyD88, IL-1β, TNF-α, while pretreatment with CLI-095 and Boxb significantly decreased the expressions of TLR4, HMGB1, MyD88, IL-1β, TNF-α. ● CONCLUSION: In A. fumigatus keratitis, Boxb play a proinflammatory role in corneal anti-fungi immune response through the HMGB1-TLR4-MyD88 signal pathway.

Original languageEnglish
Pages (from-to)548-552
Number of pages5
JournalInternational Journal of Ophthalmology
Volume11
Issue number4
DOIs
StatePublished - 2018
Externally publishedYes

Keywords

  • Aspergillus fumigatus keratitis
  • High-mobility group box 1
  • Toll-like receptor 4

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