@article{df6e86cadc5a4deabfc25b281f07f9ff,
title = "Both Neutralizing and Non-Neutralizing Human H7N9 Influenza Vaccine-Induced Monoclonal Antibodies Confer Protection",
abstract = "Pathogenic H7N9 avian influenza viruses continue to represent a public health concern, and several candidate vaccines are currently being developed. It is vital to assess if protective antibodies are induced following vaccination and to characterize the diversity of epitopes targeted. Here we characterized the binding and functional properties of twelve H7-reactive human antibodies induced by a candidate A/Anhui/1/2013 (H7N9) vaccine. Both neutralizing and non-neutralizing antibodies protected mice in vivo during passive transfer challenge experiments. Mapping the H7 hemagglutinin antigenic sites by generating escape mutant variants against the neutralizing antibodies identified unique epitopes on the head and stalk domains. Further, the broadly cross-reactive non-neutralizing antibodies generated in this study were protective through Fc-mediated effector cell recruitment. These findings reveal important properties of vaccine-induced antibodies and provide a better understanding of the human monoclonal antibody response to influenza in the context of vaccines.",
author = "{Henry Dunand}, {Carole J.} and Leon, {Paul E.} and Min Huang and Angela Choi and Veronika Chromikova and Ho, {Irvin Y.} and Tan, {Gene S.} and John Cruz and Ariana Hirsh and Zheng, {Nai Ying} and Mullarkey, {Caitlin E.} and Ennis, {Francis A.} and Masanori Terajima and Treanor, {John J.} and Topham, {David J.} and Kanta Subbarao and Peter Palese and Florian Krammer and Wilson, {Patrick C.}",
note = "Funding Information: This project has been funded in part with federal funds from the National Institute of Allergy and Infectious Diseases, NIH, Department of Health and Human Services, under CEIRS contracts No HHSN272201400006C (P.C.W., J.J.T., and D.J.T.) and HHSN272201400008C (P.P. and F.K.); NIH U19AI109946-01 (P.C.W., P.P., and F.K.), P01AI097092-03 (P.C.W. and P.P.), P01AI097092-04S1 (P.E.L.), and U19AI057266-11 (P.C.W.). Clinical trial NCT01995695 was supported by the Division of Intramural Research, NIAID, NIH and by the Biomedical Advanced Research and Development Authority, HHS, under contract #HHSN272200900026C. We thank Donna Neu, Mary Dawn T. Co, the Center for Therapeutic Antibody Development, the Flow Cytometry Center of Research Excellence, and the Microscopy Core at the Icahn School of Medicine at Mount Sinai and the University of Chicago Flow Cytometry Core. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
month = jun,
day = "8",
doi = "10.1016/j.chom.2016.05.014",
language = "English",
volume = "19",
pages = "800--813",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "6",
}