TY - JOUR
T1 - Blood-based detection of radiation exposure in humans based on novel phospho-Smc1 ELISA
AU - Ivey, Richard G.
AU - Moore, Heather D.
AU - Voytovich, Uliana J.
AU - Thienes, Cortlandt P.
AU - Lorentzen, Travis D.
AU - Pogosova-Agadjanyan, Era L.
AU - Frayo, Shani
AU - Izaguirre, Venissa K.
AU - Lundberg, Sally J.
AU - Hedin, Lacey
AU - Badiozamani, Kas Ray
AU - Hoofnagle, Andrew N.
AU - Stirewalt, Derek L.
AU - Wang, Pei
AU - Georges, George E.
AU - Gopal, Ajay K.
AU - Paulovich, Amanda G.
PY - 2011/3
Y1 - 2011/3
N2 - The structural maintenance of chromosome 1 (Smc1) protein is a member of the highly conserved cohesin complex and is involved in sister chromatid cohesion. In response to ionizing radiation, Smc1 is phosphorylated at two sites, Ser-957 and Ser-966, and these phosphorylation events are dependent on the ATM protein kinase. In this study, we describe the generation of two novel ELISAs for quantifying phospho-Smc1Ser-957 and phospho-Smc1 Ser-966. Using these novel assays, we quantify the kinetic and biodosimetric responses of human cells of hematological origin, including immortalized cells, as well as both quiescent and cycling primary human PBMC. Additionally, we demonstrate a robust in vivo response for phospho-Smc1 Ser-957 and phospho-Smc1Ser-966 in lymphocytes of human patients after therapeutic exposure to ionizing radiation, including total-body irradiation, partial-body irradiation, and internal exposure to 131I. These assays are useful for quantifying the DNA damage response in experimental systems and potentially for the identification of individuals exposed to radiation after a radiological incident.
AB - The structural maintenance of chromosome 1 (Smc1) protein is a member of the highly conserved cohesin complex and is involved in sister chromatid cohesion. In response to ionizing radiation, Smc1 is phosphorylated at two sites, Ser-957 and Ser-966, and these phosphorylation events are dependent on the ATM protein kinase. In this study, we describe the generation of two novel ELISAs for quantifying phospho-Smc1Ser-957 and phospho-Smc1 Ser-966. Using these novel assays, we quantify the kinetic and biodosimetric responses of human cells of hematological origin, including immortalized cells, as well as both quiescent and cycling primary human PBMC. Additionally, we demonstrate a robust in vivo response for phospho-Smc1 Ser-957 and phospho-Smc1Ser-966 in lymphocytes of human patients after therapeutic exposure to ionizing radiation, including total-body irradiation, partial-body irradiation, and internal exposure to 131I. These assays are useful for quantifying the DNA damage response in experimental systems and potentially for the identification of individuals exposed to radiation after a radiological incident.
UR - https://www.scopus.com/pages/publications/79952130811
U2 - 10.1667/RR2402.1
DO - 10.1667/RR2402.1
M3 - Article
C2 - 21388270
AN - SCOPUS:79952130811
SN - 0033-7587
VL - 175
SP - 266
EP - 281
JO - Radiation Research
JF - Radiation Research
IS - 3
ER -