Block-copolymer micelles as carriers of cell signaling modulators for the inhibition of JNK in human islets of Langerhans

Radoslav Savić, Tony Azzam, Adi Eisenberg, Hinyu Nedev, Lawrence Rosenberg, Dusica Maysinger

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Here we investigate the potential of PCL-b-PEO micelles in preventing the cell death of isolated human islets of Langerhans. PCL-b-PEO micelles were loaded with c-Jun NH2-terminal kinases inhibitor SP600125 to rescue the isolated islets. Mechanistic studies of the uptake were conducted in PC12 cells. Incorporation of SP600125 afforded 8.2 fold greater solubility of SP600125 in micelle suspension. To investigate the effectiveness of micelle-incorporated SP600125 in preventing the islet cell death, we challenged the islets with TNF-alpha, IL-1, and IFN gamma. Micelle-incorporated SP600125 did not lose its inhibitory activity during incorporation into micelles, and it protected the islets against cytokine-induced loss of viability to the same extent as control SP600125. Moreover, the concentration of micelle-incorporated SP600125 used was 13-fold lower, demonstrating the greater efficacy of micelle delivered SP600125. Micelles maintained their cytoplasmic distribution without detectable nuclear localization in islets. The inhibition of JNK was confirmed by western blots. This study suggests that micelle-based intracellular delivery of potent, poorly water soluble, cell-death-pathway inhibitors may represent a valuable addition to established delivery of cytocidal block-copolymer micelle-incorporated bioactives.

Original languageEnglish
Pages (from-to)3597-3604
Number of pages8
Issue number21
StatePublished - Jul 2009
Externally publishedYes


  • Diabetes
  • Drug delivery
  • Islet
  • Micelle
  • Polycaprolactone
  • Polyethyleneoxide


Dive into the research topics of 'Block-copolymer micelles as carriers of cell signaling modulators for the inhibition of JNK in human islets of Langerhans'. Together they form a unique fingerprint.

Cite this