TY - JOUR
T1 - Biotyping and exoenzyme profiling as an aid in the differentiation of human from bovine group G streptococci
AU - Clark, R. B.
AU - Berrafati, J. F.
AU - Janda, J. M.
AU - Bottone, E. J.
PY - 1984
Y1 - 1984
N2 - Group G streptococci were isolated from throat and extrapharyngeal cultures from 75 patients during an 18-month period. Of 29 throat isolates, 18 were recovered from patients with pharyngitis, 8 were of unknown significance, and 3 were of questionable etiology, Clinical significance could be ascribed to 13 of 46 extrapharyngeal isolates recovered from wound, urinary tract, blood, and conjunctival cultures. Extrapharyngeal isolates recovered from stool, sputum, and vaginal cultures were considered nonsignificant. A total of 96 group G streptococcal strains (including 21 human and 14 bovine strains from outside sources) were tested for exoenzyme production and subjected to a large battery of biochemical tests. Bovine and human isolates could be distinguished on the basis of trehalose fermentation, litmus milk reduction, and production of β-D-glucuronidase, hyaluronidase, and fibrinolysin. Eight distinct biotypes could be discerned on the basis of fermentation of trehalose, raffinose, and lactose and esculin hydrolysis. All isolates that fermented raffinose were associated with infection. These results support the concept of two distinctly different epidemiological reservoirs of group G streptococci in humans and bovines.
AB - Group G streptococci were isolated from throat and extrapharyngeal cultures from 75 patients during an 18-month period. Of 29 throat isolates, 18 were recovered from patients with pharyngitis, 8 were of unknown significance, and 3 were of questionable etiology, Clinical significance could be ascribed to 13 of 46 extrapharyngeal isolates recovered from wound, urinary tract, blood, and conjunctival cultures. Extrapharyngeal isolates recovered from stool, sputum, and vaginal cultures were considered nonsignificant. A total of 96 group G streptococcal strains (including 21 human and 14 bovine strains from outside sources) were tested for exoenzyme production and subjected to a large battery of biochemical tests. Bovine and human isolates could be distinguished on the basis of trehalose fermentation, litmus milk reduction, and production of β-D-glucuronidase, hyaluronidase, and fibrinolysin. Eight distinct biotypes could be discerned on the basis of fermentation of trehalose, raffinose, and lactose and esculin hydrolysis. All isolates that fermented raffinose were associated with infection. These results support the concept of two distinctly different epidemiological reservoirs of group G streptococci in humans and bovines.
UR - http://www.scopus.com/inward/record.url?scp=0021175792&partnerID=8YFLogxK
U2 - 10.1128/jcm.20.4.706-710.1984
DO - 10.1128/jcm.20.4.706-710.1984
M3 - Article
C2 - 6238045
AN - SCOPUS:0021175792
SN - 0095-1137
VL - 20
SP - 706
EP - 710
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 4
ER -