Biosynthesis of the identical form of monooxygenase induced by xenobiotics of the MC-type in rat liver

I. B. Tsyrlov, O. B. Chasovnikova, A. Grishanova Yu., V. V. Lyakhovich

Research output: Contribution to journalArticlepeer-review

Abstract

Injection of Wistar rats with five structurally different inducers of methylcholanthrene-type (polycyclic aromatic and heterocyclic hydrocarbons, chloro-derivatives of biphenyl and dibenzo-p-dioxin) results in the de novo synthesis of two P-448 hemoproteins (molecular weight 56,000 and 53,000 Da) differing in their functional and immunochemical properties in liver microsomes. A comparison of catalytic and immunochemical characteristics of five cytochrome P-448 forms (M(r) = 56,000 Da) as well as the data from electrophoretic, proteolytic and inhibitory analyses revealed no differences in the preparations used, with the exception of 2,3,7,8-tetrachloro-dibenzo-p-dioxin-induced microsomes characterized by a low level of this cytochrome P-448 form and a higher molecular activity as compared with 3,4-benzypyrene and 7-ethoxyresorufin-induced microsomes. The experimental results do not confirm the hypothesis on the feasibility of induced synthesis of a variety of individual forms of monooxygenase that would correlate with the number of structural variants of inducers.

Original languageEnglish
Pages (from-to)579-589
Number of pages11
JournalBiokhimiya
Volume51
Issue number4
StatePublished - 1986
Externally publishedYes

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