Biosensors from conjugated polyelectrolyte complexes

Deli Wang; Xiong Gong; Peter S. Heeger; Frauke Rininsland; Guillermo C. Bazan; Alan J. Heeger

doi:10.1073/pnas.012581399

Biosensors from conjugated polyelectrolyte complexes

Deli Wang, Xiong Gong, Peter S. Heeger, Frauke Rininsland, Guillermo C. Bazan, Alan J. Heeger

Research output: Contribution to journal › Article › peer-review

293 Scopus citations

Abstract

A charge neutral complex (CNC) was formed in aqueous solution by combining an orange light emitting anionic conjugated polyelectrolyte and a saturated cationic polyelectrolyte at a 1:1 ratio (per repeat unit). Photoluminescence (PL) from the CNC can be quenched by both the negatively charged dinitrophenol (DNP) derivative, (DNP-BS^-), and positively charged methyl viologen (MV²⁺). Use of the CNC minimizes nonspecific interactions (which modify the PL) between conjugated polyelectrolytes and biopolymers. Quenching of the PL from the CNC by the DNP derivative and specific unquenching on addition of anti-DNP antibody (anti-DNP IgG) were observed. Thus, biosensing of the anti-DNP IgG was demonstrated.

Original language	English
Pages (from-to)	49-53
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Issue number	1
DOIs	https://doi.org/10.1073/pnas.012581399
State	Published - 8 Jan 2002
Externally published	Yes

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abstract = "A charge neutral complex (CNC) was formed in aqueous solution by combining an orange light emitting anionic conjugated polyelectrolyte and a saturated cationic polyelectrolyte at a 1:1 ratio (per repeat unit). Photoluminescence (PL) from the CNC can be quenched by both the negatively charged dinitrophenol (DNP) derivative, (DNP-BS-), and positively charged methyl viologen (MV2+). Use of the CNC minimizes nonspecific interactions (which modify the PL) between conjugated polyelectrolytes and biopolymers. Quenching of the PL from the CNC by the DNP derivative and specific unquenching on addition of anti-DNP antibody (anti-DNP IgG) were observed. Thus, biosensing of the anti-DNP IgG was demonstrated.",

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T1 - Biosensors from conjugated polyelectrolyte complexes

AU - Wang, Deli

AU - Gong, Xiong

AU - Heeger, Peter S.

AU - Rininsland, Frauke

AU - Bazan, Guillermo C.

AU - Heeger, Alan J.

PY - 2002/1/8

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N2 - A charge neutral complex (CNC) was formed in aqueous solution by combining an orange light emitting anionic conjugated polyelectrolyte and a saturated cationic polyelectrolyte at a 1:1 ratio (per repeat unit). Photoluminescence (PL) from the CNC can be quenched by both the negatively charged dinitrophenol (DNP) derivative, (DNP-BS-), and positively charged methyl viologen (MV2+). Use of the CNC minimizes nonspecific interactions (which modify the PL) between conjugated polyelectrolytes and biopolymers. Quenching of the PL from the CNC by the DNP derivative and specific unquenching on addition of anti-DNP antibody (anti-DNP IgG) were observed. Thus, biosensing of the anti-DNP IgG was demonstrated.

AB - A charge neutral complex (CNC) was formed in aqueous solution by combining an orange light emitting anionic conjugated polyelectrolyte and a saturated cationic polyelectrolyte at a 1:1 ratio (per repeat unit). Photoluminescence (PL) from the CNC can be quenched by both the negatively charged dinitrophenol (DNP) derivative, (DNP-BS-), and positively charged methyl viologen (MV2+). Use of the CNC minimizes nonspecific interactions (which modify the PL) between conjugated polyelectrolytes and biopolymers. Quenching of the PL from the CNC by the DNP derivative and specific unquenching on addition of anti-DNP antibody (anti-DNP IgG) were observed. Thus, biosensing of the anti-DNP IgG was demonstrated.

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Biosensors from conjugated polyelectrolyte complexes

Abstract

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