Biological properties of a renotropic protein present in plasma of uninephrectomized rats

P. Esbrit; A. Garcia-Ocana; R. Garcia-Canero; F. Manzano; M. A. Jimenez-Clavero

Biological properties of a renotropic protein present in plasma of uninephrectomized rats

P. Esbrit
, A. Garcia-Ocana
, R. Garcia-Canero
, F. Manzano
, M. A. Jimenez-Clavero

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The biological characteristics of a kidney growth factor (KGF) from uninephrectomized rat plasma have been studied. A crude preparation of this factor [Nephrol. Dial. Transplant. 4:334-338,1989] was further purified by hydrophobic interaction HPLC and gel filtration. KGF was found to be a heat- and trypsin-resistant protein. This factor stimulated dose-dependently DNA synthesis by the mouse kidney in vivo, and by either rat renal tubules or serum-deprived LLC-PK₁ cells, in vitro. KGF also increased protein synthesis in these cells, in a dose-dependent manner. Moreover, KGF stimulated sodium uptake by these cells, associated with the maximal increase of protein synthesis. Our findings indicate that KGF is a potent renotropic protein which can play a key role in the renal compensatory growth after uninephrectomy.

Original language	English
Pages (from-to)	224-235
Number of pages	12
Journal	Renal Physiology and Biochemistry
Volume	14
Issue number	6
State	Published - 1991
Externally published	Yes

Keywords

Kidney growth factor
Renal compensatory growth
Renotropic protein
Uninephrectomy

Cite this

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title = "Biological properties of a renotropic protein present in plasma of uninephrectomized rats",

abstract = "The biological characteristics of a kidney growth factor (KGF) from uninephrectomized rat plasma have been studied. A crude preparation of this factor [Nephrol. Dial. Transplant. 4:334-338,1989] was further purified by hydrophobic interaction HPLC and gel filtration. KGF was found to be a heat- and trypsin-resistant protein. This factor stimulated dose-dependently DNA synthesis by the mouse kidney in vivo, and by either rat renal tubules or serum-deprived LLC-PK1 cells, in vitro. KGF also increased protein synthesis in these cells, in a dose-dependent manner. Moreover, KGF stimulated sodium uptake by these cells, associated with the maximal increase of protein synthesis. Our findings indicate that KGF is a potent renotropic protein which can play a key role in the renal compensatory growth after uninephrectomy.",

keywords = "Kidney growth factor, Renal compensatory growth, Renotropic protein, Uninephrectomy",

author = "P. Esbrit and A. Garcia-Ocana and R. Garcia-Canero and F. Manzano and Jimenez-Clavero, \{M. A.\}",

year = "1991",

language = "English",

volume = "14",

pages = "224--235",

journal = "Renal Physiology and Biochemistry",

issn = "1011-6524",

publisher = "S. Karger AG",

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}

TY - JOUR

T1 - Biological properties of a renotropic protein present in plasma of uninephrectomized rats

AU - Esbrit, P.

AU - Garcia-Ocana, A.

AU - Garcia-Canero, R.

AU - Manzano, F.

AU - Jimenez-Clavero, M. A.

PY - 1991

Y1 - 1991

N2 - The biological characteristics of a kidney growth factor (KGF) from uninephrectomized rat plasma have been studied. A crude preparation of this factor [Nephrol. Dial. Transplant. 4:334-338,1989] was further purified by hydrophobic interaction HPLC and gel filtration. KGF was found to be a heat- and trypsin-resistant protein. This factor stimulated dose-dependently DNA synthesis by the mouse kidney in vivo, and by either rat renal tubules or serum-deprived LLC-PK1 cells, in vitro. KGF also increased protein synthesis in these cells, in a dose-dependent manner. Moreover, KGF stimulated sodium uptake by these cells, associated with the maximal increase of protein synthesis. Our findings indicate that KGF is a potent renotropic protein which can play a key role in the renal compensatory growth after uninephrectomy.

AB - The biological characteristics of a kidney growth factor (KGF) from uninephrectomized rat plasma have been studied. A crude preparation of this factor [Nephrol. Dial. Transplant. 4:334-338,1989] was further purified by hydrophobic interaction HPLC and gel filtration. KGF was found to be a heat- and trypsin-resistant protein. This factor stimulated dose-dependently DNA synthesis by the mouse kidney in vivo, and by either rat renal tubules or serum-deprived LLC-PK1 cells, in vitro. KGF also increased protein synthesis in these cells, in a dose-dependent manner. Moreover, KGF stimulated sodium uptake by these cells, associated with the maximal increase of protein synthesis. Our findings indicate that KGF is a potent renotropic protein which can play a key role in the renal compensatory growth after uninephrectomy.

KW - Kidney growth factor

KW - Renal compensatory growth

KW - Renotropic protein

KW - Uninephrectomy

UR - https://www.scopus.com/pages/publications/0026077107

M3 - Article

C2 - 1720253

AN - SCOPUS:0026077107

SN - 1011-6524

VL - 14

SP - 224

EP - 235

JO - Renal Physiology and Biochemistry

JF - Renal Physiology and Biochemistry

IS - 6

ER -

Biological properties of a renotropic protein present in plasma of uninephrectomized rats

Abstract

Keywords

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