TY - JOUR
T1 - Biological properties of a renotropic protein present in plasma of uninephrectomized rats
AU - Esbrit, P.
AU - Garcia-Ocana, A.
AU - Garcia-Canero, R.
AU - Manzano, F.
AU - Jimenez-Clavero, M. A.
PY - 1991
Y1 - 1991
N2 - The biological characteristics of a kidney growth factor (KGF) from uninephrectomized rat plasma have been studied. A crude preparation of this factor [Nephrol. Dial. Transplant. 4:334-338,1989] was further purified by hydrophobic interaction HPLC and gel filtration. KGF was found to be a heat- and trypsin-resistant protein. This factor stimulated dose-dependently DNA synthesis by the mouse kidney in vivo, and by either rat renal tubules or serum-deprived LLC-PK1 cells, in vitro. KGF also increased protein synthesis in these cells, in a dose-dependent manner. Moreover, KGF stimulated sodium uptake by these cells, associated with the maximal increase of protein synthesis. Our findings indicate that KGF is a potent renotropic protein which can play a key role in the renal compensatory growth after uninephrectomy.
AB - The biological characteristics of a kidney growth factor (KGF) from uninephrectomized rat plasma have been studied. A crude preparation of this factor [Nephrol. Dial. Transplant. 4:334-338,1989] was further purified by hydrophobic interaction HPLC and gel filtration. KGF was found to be a heat- and trypsin-resistant protein. This factor stimulated dose-dependently DNA synthesis by the mouse kidney in vivo, and by either rat renal tubules or serum-deprived LLC-PK1 cells, in vitro. KGF also increased protein synthesis in these cells, in a dose-dependent manner. Moreover, KGF stimulated sodium uptake by these cells, associated with the maximal increase of protein synthesis. Our findings indicate that KGF is a potent renotropic protein which can play a key role in the renal compensatory growth after uninephrectomy.
KW - Kidney growth factor
KW - Renal compensatory growth
KW - Renotropic protein
KW - Uninephrectomy
UR - http://www.scopus.com/inward/record.url?scp=0026077107&partnerID=8YFLogxK
M3 - Article
C2 - 1720253
AN - SCOPUS:0026077107
SN - 1011-6524
VL - 14
SP - 224
EP - 235
JO - Renal Physiology and Biochemistry
JF - Renal Physiology and Biochemistry
IS - 6
ER -