Biological activity of calcitonin and its assessment

I. MacIntyre, A. Kehely, M. Zaidi, R. Seth

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Radioimmunoassay has been method of choice for the routine determination of calcitonin (CT) in biological fluids, mainly due to its precision, sensitivity, and ability to handle large numbers of samples. However, spuriously high results are often obtained due to undefined cross-reactivities of polyclonal antisera to precursors, polymers, metabolites, or fragments of CT, or to other physiological and pathological variants of the hormone. In routine practice, radioimmunoassays of CT are being replaced by immunometric two-site 'sandwich' assays utilizing monoclonal antiboies against defined antigenic epitopes. Hormone activity is, however, accurately quantifiable by biological assays based on the exquisite hormonal sensitivity of isolated osteoclasts. The assays are, highly specific and remarkably sensitive. Their use in routine laboratories is nevertheless limited only to specific situations.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalExperimental Gerontology
Issue number3-4
StatePublished - 1990
Externally publishedYes


  • immunoassay
  • peptide
  • thyroid


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