Bioengineered acellular dermal matrix for the repair of full thickness skin wounds in rats

Anil K. Gangwar; Naveen Kumar; Ashok K. Sharma; Kh Sangeeta Devi; Mamta Negi; Sameer Shrivastava; Dayamon D. Mathew; V. Remya; Sonal; P. S. Arundeep; Swapan K. Maiti; Vineet Kumar; D. T. Kaarthick; N. P. Kurade; Rajendra Singh

Bioengineered acellular dermal matrix for the repair of full thickness skin wounds in rats

Anil K. Gangwar
, Naveen Kumar
, Ashok K. Sharma
, Kh Sangeeta Devi
, Mamta Negi
, Sameer Shrivastava
, Dayamon D. Mathew
, V. Remya
, Sonal
, P. S. Arundeep
, Swapan K. Maiti
, Vineet Kumar
, D. T. Kaarthick
, N. P. Kurade
, Rajendra Singh

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The rat skin was de-epithelialized using hypertonic saline solution and further decellularized with 1% sodium deoxycholate. The primary mouse embryonic fibroblast (P-MEF) cells were seeded on acellular dermal matrix (ADM). Fortyfive rats were randomly divided into 3 groups of 15 animals each. A 20×20mm² full thickness skin defect was created on dorsum. The defect in group-I was dressed with standard dressing material, group-II with ADM, and group-III ADM seeded with P-MEF. In group-III, complete healing was observed at day21. The values of hydroxyproline contents in group III were significantly higher on days14 and 21 as compared to group-I. Significant decrease in glucosamine content was observed up to day28 in group-III. Native graft antigen challenged animals showed highest B-cell response. The acellular graft antigen challenged animals showed no B-cell response. Biopsy specimens showed minimum histopathological scores in group-III. The level of MDA and SOD increased significantly up to day14 in group-I. On day3, significantly lower values of GSH were observed in group-I whereas, significantly higher values of catalase were observed in all groups. SEM examination revealed well spread fibroblasts over the scaffold in group-III. These results indicate that bioengineered grafts can be used clinically to manage full thickness skin wounds.

Original language	English
Pages (from-to)	67-80
Number of pages	14
Journal	Trends in Biomaterials and Artificial Organs
Volume	27
Issue number	2
State	Published - 2013
Externally published	Yes

Cite this

@article{6c0fc5546e5b45258f4adb2c72a6f040,

title = "Bioengineered acellular dermal matrix for the repair of full thickness skin wounds in rats",

abstract = "The rat skin was de-epithelialized using hypertonic saline solution and further decellularized with 1\% sodium deoxycholate. The primary mouse embryonic fibroblast (P-MEF) cells were seeded on acellular dermal matrix (ADM). Fortyfive rats were randomly divided into 3 groups of 15 animals each. A 20{\texttimes}20mm2 full thickness skin defect was created on dorsum. The defect in group-I was dressed with standard dressing material, group-II with ADM, and group-III ADM seeded with P-MEF. In group-III, complete healing was observed at day21. The values of hydroxyproline contents in group III were significantly higher on days14 and 21 as compared to group-I. Significant decrease in glucosamine content was observed up to day28 in group-III. Native graft antigen challenged animals showed highest B-cell response. The acellular graft antigen challenged animals showed no B-cell response. Biopsy specimens showed minimum histopathological scores in group-III. The level of MDA and SOD increased significantly up to day14 in group-I. On day3, significantly lower values of GSH were observed in group-I whereas, significantly higher values of catalase were observed in all groups. SEM examination revealed well spread fibroblasts over the scaffold in group-III. These results indicate that bioengineered grafts can be used clinically to manage full thickness skin wounds.",

author = "Gangwar, \{Anil K.\} and Naveen Kumar and Sharma, \{Ashok K.\} and Devi, \{Kh Sangeeta\} and Mamta Negi and Sameer Shrivastava and Mathew, \{Dayamon D.\} and V. Remya and Sonal and Arundeep, \{P. S.\} and Maiti, \{Swapan K.\} and Vineet Kumar and Kaarthick, \{D. T.\} and Kurade, \{N. P.\} and Rajendra Singh",

year = "2013",

language = "English",

volume = "27",

pages = "67--80",

journal = "Trends in Biomaterials and Artificial Organs",

issn = "0971-1198",

publisher = "Society for Biomaterials and Artificial Organs - India",

number = "2",

}

TY - JOUR

T1 - Bioengineered acellular dermal matrix for the repair of full thickness skin wounds in rats

AU - Gangwar, Anil K.

AU - Kumar, Naveen

AU - Sharma, Ashok K.

AU - Devi, Kh Sangeeta

AU - Negi, Mamta

AU - Shrivastava, Sameer

AU - Mathew, Dayamon D.

AU - Remya, V.

AU - Sonal,

AU - Arundeep, P. S.

AU - Maiti, Swapan K.

AU - Kumar, Vineet

AU - Kaarthick, D. T.

AU - Kurade, N. P.

AU - Singh, Rajendra

PY - 2013

Y1 - 2013

N2 - The rat skin was de-epithelialized using hypertonic saline solution and further decellularized with 1% sodium deoxycholate. The primary mouse embryonic fibroblast (P-MEF) cells were seeded on acellular dermal matrix (ADM). Fortyfive rats were randomly divided into 3 groups of 15 animals each. A 20×20mm2 full thickness skin defect was created on dorsum. The defect in group-I was dressed with standard dressing material, group-II with ADM, and group-III ADM seeded with P-MEF. In group-III, complete healing was observed at day21. The values of hydroxyproline contents in group III were significantly higher on days14 and 21 as compared to group-I. Significant decrease in glucosamine content was observed up to day28 in group-III. Native graft antigen challenged animals showed highest B-cell response. The acellular graft antigen challenged animals showed no B-cell response. Biopsy specimens showed minimum histopathological scores in group-III. The level of MDA and SOD increased significantly up to day14 in group-I. On day3, significantly lower values of GSH were observed in group-I whereas, significantly higher values of catalase were observed in all groups. SEM examination revealed well spread fibroblasts over the scaffold in group-III. These results indicate that bioengineered grafts can be used clinically to manage full thickness skin wounds.

AB - The rat skin was de-epithelialized using hypertonic saline solution and further decellularized with 1% sodium deoxycholate. The primary mouse embryonic fibroblast (P-MEF) cells were seeded on acellular dermal matrix (ADM). Fortyfive rats were randomly divided into 3 groups of 15 animals each. A 20×20mm2 full thickness skin defect was created on dorsum. The defect in group-I was dressed with standard dressing material, group-II with ADM, and group-III ADM seeded with P-MEF. In group-III, complete healing was observed at day21. The values of hydroxyproline contents in group III were significantly higher on days14 and 21 as compared to group-I. Significant decrease in glucosamine content was observed up to day28 in group-III. Native graft antigen challenged animals showed highest B-cell response. The acellular graft antigen challenged animals showed no B-cell response. Biopsy specimens showed minimum histopathological scores in group-III. The level of MDA and SOD increased significantly up to day14 in group-I. On day3, significantly lower values of GSH were observed in group-I whereas, significantly higher values of catalase were observed in all groups. SEM examination revealed well spread fibroblasts over the scaffold in group-III. These results indicate that bioengineered grafts can be used clinically to manage full thickness skin wounds.

UR - https://www.scopus.com/pages/publications/84888219678

M3 - Article

AN - SCOPUS:84888219678

SN - 0971-1198

VL - 27

SP - 67

EP - 80

JO - Trends in Biomaterials and Artificial Organs

JF - Trends in Biomaterials and Artificial Organs

IS - 2

ER -

Bioengineered acellular dermal matrix for the repair of full thickness skin wounds in rats

Abstract

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