Biodistribution studies of anti-Thy 1.2 IgM immunoconjugates: Implications for radioimmunotherapy

Roger M. Macklis, William D. Kaplan, James L.M. Ferrara, Berma M. Kinsey, Amin I. Kassis, Steven J. Burakoff

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


We have prepared 111In radioimmunoconjugates (RICs) of the IgM isotype with specificity for the murine T cell/neuroectgdermal surface antigen, Thy 1.2. Using gamma camera immunoscintigraphy, we have analyzed the biodistribution patterns of the RICs after intravenous and intraperitoneal injection into normal Thy 1.2+ and Thy 1.2- mice. Both routes of administration show antigen-specific uptake by the splenic T lymphocyte population. A high degree of nonspecific uptake by the reticuloendothelial system is also observed. Analysis of the specific activity of various segments of spleens from RIC-injected animals shows inhomogeneous uptake of the RIC not readily apparent by immunoscintigraphy. Animals injected with the RIC and then given high dose total body irradiation showed rapid shifts in radionuclide distribution away from the target cell population and into the general reticuloendothelial system, suggesting that death of the target cell can alter RIC biodistribution. Analyses of RIC biodistribution patterns will contribute to optimization of treatment by radioimmunotherapy.

Original languageEnglish
Pages (from-to)383-389
Number of pages7
JournalInternational Journal of Radiation Oncology Biology Physics
Issue number2
StatePublished - Aug 1988
Externally publishedYes


  • DTPA
  • IgM
  • Immunoscintigraphy
  • Radioimmunotherapy
  • Thy 1


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