Biochemical changes in cultured foetal rat liver explants

P. C. MacDonnell, E. Ryder, J. A. Delvalle, O. Greengard

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Liver explants from 20 day old fetuses cultured for 48 hr in the absence of serum released 70% of their total soluble protein content into the medium. In the presence of serum this loss still amounted to 60%. The concentration of total particulate protein remained unchanged but there was some translocation of mitochondrial enzymes to the cytosol, and enzymes expected to increase during this stage of development failed to do so. The addition of cortisol plus glucagon (to serum containing media) did not decrease the loss of total soluble protein from the explants but induced considerable tyrosine aminotransferase activity which was not released into the medium. The observations suggest that under the usual culture conditions a minority of the cells retain their functional integrity. The extent of deterioration, not reflected in histologically visible necrosis or cell damage, can be conveniently monitored by the malate dehydrogenase activity released to the medium.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalBiochemical Journal
Issue number2
StatePublished - 1975
Externally publishedYes


Dive into the research topics of 'Biochemical changes in cultured foetal rat liver explants'. Together they form a unique fingerprint.

Cite this