TY - JOUR
T1 - Bile Microbiota in Liver Transplantation
T2 - Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
AU - D'Amico, Francesco
AU - Bertacco, Alessandra
AU - Finotti, Michele
AU - Di Renzo, Chiara
AU - Rodriguez-Davalos, Manuel I.
AU - Gondolesi, Gabriel E.
AU - Cillo, Umberto
AU - Mulligan, David
AU - Geibel, John
N1 - Publisher Copyright:
© Copyright © 2021 D'Amico, Bertacco, Finotti, Di Renzo, Rodriguez-Davalos, Gondolesi, Cillo, Mulligan and Geibel.
PY - 2021/3/16
Y1 - 2021/3/16
N2 - Objective: Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. Methods: We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients, with samples obtained in a sterile way, directly from the gallbladder and the common bile duct. Results: We assessed the bile microbiota in six liver donors and in six liver recipients after whole or split liver procedures in adult or pediatric recipients. Bile samples were studied using PCR sequencing of the 16S ribosomal RNA gene amplification (rDNA). Conclusions: We demonstrated that the bile is sterile, thereby ruling this out as a source of contamination following transplant.
AB - Objective: Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. Methods: We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients, with samples obtained in a sterile way, directly from the gallbladder and the common bile duct. Results: We assessed the bile microbiota in six liver donors and in six liver recipients after whole or split liver procedures in adult or pediatric recipients. Bile samples were studied using PCR sequencing of the 16S ribosomal RNA gene amplification (rDNA). Conclusions: We demonstrated that the bile is sterile, thereby ruling this out as a source of contamination following transplant.
KW - 16S ribosomal RNA gene amplification
KW - biliary infections
KW - gallbladder
KW - microbiome
KW - rDNA sequence analysis
UR - http://www.scopus.com/inward/record.url?scp=85103487440&partnerID=8YFLogxK
U2 - 10.3389/fsurg.2021.621525
DO - 10.3389/fsurg.2021.621525
M3 - Article
AN - SCOPUS:85103487440
SN - 2296-875X
VL - 8
JO - Frontiers in Surgery
JF - Frontiers in Surgery
M1 - 621525
ER -