TY - JOUR
T1 - BglII and MunI
T2 - What a difference a base makes
AU - Lukacs, Christine M.
AU - Aggarwal, Aneel K.
N1 - Funding Information:
We thank Ira Schildkraut and Steve Halford for many stimulating discussions, and Jose Trincao for help with the figures. AKA is supported by a grant from the National Institutes of Health.
PY - 2001/2/1
Y1 - 2001/2/1
N2 - Restriction endonucleases are resilient to alterations in their DNA-binding specificities. Structures of the BglII and MunI endonucleases bound to their palindromic DNA sites, which differ by only their outer base pairs from the recognition sequences of BamHI and EcoRI, respectively, have recently been determined. A comparison of these complexes reveals surprising differences and similarities in structure, and provides a basis for understanding the immutability of restriction endonucleases.
AB - Restriction endonucleases are resilient to alterations in their DNA-binding specificities. Structures of the BglII and MunI endonucleases bound to their palindromic DNA sites, which differ by only their outer base pairs from the recognition sequences of BamHI and EcoRI, respectively, have recently been determined. A comparison of these complexes reveals surprising differences and similarities in structure, and provides a basis for understanding the immutability of restriction endonucleases.
UR - http://www.scopus.com/inward/record.url?scp=0035252681&partnerID=8YFLogxK
U2 - 10.1016/S0959-440X(00)00174-3
DO - 10.1016/S0959-440X(00)00174-3
M3 - Review article
C2 - 11179886
AN - SCOPUS:0035252681
SN - 0959-440X
VL - 11
SP - 14
EP - 18
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 1
ER -