Axo-glial interactions between midbrain dopamine neurons and oligodendrocyte lineage cells in the anterior corpus callosum

Megan Caldwell, Vanessa Ayo-Jibunoh, Josue Criollo Mendoza, Katherine R. Brimblecombe, Lauren M. Reynolds, Xin Yan Zhu Jiang, Colin Alarcon, Elizabeth Fiore, Jacquelyn N. Tomaio, Greg R. Phillips, Susana Mingote, Cecilia Flores, Patrizia Casaccia, Jia Liu, Stephanie J. Cragg, Dan P. McCloskey, Leora Yetnikoff

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Oligodendrocyte progenitor cells (OPCs) receive synaptic innervation from glutamatergic and GABAergic axons and can be dynamically regulated by neural activity, resulting in activity-dependent changes in patterns of axon myelination. However, it remains unclear to what extent other types of neurons may innervate OPCs. Here, we provide evidence implicating midbrain dopamine neurons in the innervation of oligodendrocyte lineage cells in the anterior corpus callosum and nearby white matter tracts of male and female adult mice. Dopaminergic axon terminals were identified in the corpus callosum of DAT-Cre mice after injection of an eYFP reporter virus into the midbrain. Furthermore, fast-scan cyclic voltammetry revealed monoaminergic transients in the anterior corpus callosum, consistent with the anatomical findings. Using RNAscope, we further demonstrate that ~ 40% of Olig2 + /Pdfgra + cells and ~ 20% of Olig2 + /Pdgfra- cells in the anterior corpus callosum express Drd1 and Drd2 transcripts. These results suggest that oligodendrocyte lineage cells may respond to dopamine released from midbrain dopamine axons, which could affect myelination. Together, this work broadens our understanding of neuron-glia interactions with important implications for myelin plasticity by identifying midbrain dopamine axons as a potential regulator of corpus callosal oligodendrocyte lineage cells.

Original languageEnglish
Pages (from-to)1993-2006
Number of pages14
JournalBrain Structure and Function
Volume228
Issue number8
DOIs
StatePublished - Nov 2023
Externally publishedYes

Keywords

  • Dopamine d1 receptor
  • Dopamine d2 receptor
  • Dopamine-glial interactions
  • Myelin plasticity
  • Oligodendrocyte progenitor cells

Fingerprint

Dive into the research topics of 'Axo-glial interactions between midbrain dopamine neurons and oligodendrocyte lineage cells in the anterior corpus callosum'. Together they form a unique fingerprint.

Cite this