Automated Detection of Psychedelic-Induced Head-Twitch Response in Mice

Alaina M. Jaster, Javier González-Maeso

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Head-twitch response (HTR) allows for the detection and classification of behavior associated with serotonin 2A receptor (5-HT2AR) activation upon psychedelic administration in rodent models. This activation and functional output can be utilized to provide insights into molecular mechanisms associated with psychosis and to identify signaling processes related to existing and novel antipsychotic and psychedelic compounds. Here we describe the use of a magnetic ear tag reporter coupled with automated quantification and biphasic detection to identify HTR in mice treated with the classical psychedelic 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages65-76
Number of pages12
DOIs
StatePublished - 2023
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2687
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Behavioral model
  • Hallucinogen
  • Head-twitch response (HTR)
  • Preclinical
  • Psychedelics
  • Psychosis
  • Rodent models
  • Schizophrenia
  • Serotonin 2A receptor (5-HTR)

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