Autoantibody-associated cross-reactive idiotype-bearing human B lymphocytes: Distribution and characterization, including Ig V(H) gene and CD5 antigen expression

G. Inghirami, D. R. Foitl, A. Sabichi, B. Y. Zhu, D. M. Knowles

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Monoclonal antibodies (MoAbs) specific for autoantibody associated cross-reactive idiotypes (CRIs) frequently recognize the Igs of neoplastic B cells in patients with chronic lymphocytic leukemia (CLL) and/or Waldenstrom's macroglobulinemia. Very little is known regarding the normal B cells expressing CRIs (CRI-positive B cells). Using a variety of MoAbs against CRIs we investigated the distribution and topographic localization of CRI-positive B cells in normal adult human lymphoid tissues. We found that CRI-positive B cells represent a significant B-cell subpopulation expressing surface IgM (>90%), IgG (~5%), or IgA (~2%). CRI-positive B cells are homogeneously distributed throughout all lymphoid tissues, accounting for 10% to 15% of all B lymphocytes, with the exception of the thymus, in which they represent the predominant B cell population. Immunophenotypic studies showed (1) that a small subpopulation (3.7% ± 0.8%) of CRI-positive B cells are activated in vivo, based on CD25 and CD38 antigen expression; and (2) that approximately 50% of CRI-positive B cells express the 67-Kd pan-T-lymphocyte CD5 antigen, suggesting that the CRI-positive B-cell subset and the recently described CD5-positive B-cell subset are closely related. This hypothesis is supported by the fact that CRI-positive B cells produce oligo or polyreactive Igs, which are a characteristic feature of CD5-positive B cells, and also by the fact that both B-cell subpopulations appear to use similar and restricted Ig V(H) gene family members.

Original languageEnglish
Pages (from-to)1503-1515
Number of pages13
JournalBlood
Volume78
Issue number6
DOIs
StatePublished - 1991
Externally publishedYes

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