Autoantibodies against cancer antigens

Sacha Gnjatic, Lloyd J. Old, Yao Tseng Chen

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

45 Scopus citations

Abstract

With the identification of tumor antigens, the host immune response to various types of cancers can now be studied with a high degree of specificity in large cohorts of patients, in the hope of correlating immunity with clinical events and defining immunotherapeutic strategies. Several antigens, such as NY-ESO-1, p53, or SOX2, have attracted attention because of their frequent spontaneous immunogenicity in cancer patients, notably in their ability to induce humoral responses. We describe in this chapter a simple implementation of a serological monitoring platform for autoantibody measurement in cancer patients, from production of recombinant antigens to ELISA testing and interpretation.

Original languageEnglish
Title of host publicationTumor Biomarker Discovery
Subtitle of host publicationMethods and Protocols
EditorsMichael Tainsky
Pages11-19
Number of pages9
DOIs
StatePublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume520
ISSN (Print)1064-3745

Keywords

  • Antibody titration and titer calculation
  • Enzyme-linked immunosorbent assay
  • Humoral immunity
  • Immunoediting
  • Immunoglobulin (IgG, IgM, IgG1, IgG2, IgG3, IgG4)
  • Immunosurveillance
  • Plasma
  • Protein purification
  • Serological screening
  • Serum
  • Tumor antigen

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