Attempts to detect virus-specific DNA sequences in human tumors - III. Epstein-Barr viral DNA in non-lymphoid nasopharyngeal carcinoma cells

Hans Wolf, Harald zur Hausen, George Klein, Volker Becker, Gertrude Henle, Werner Henle

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Fourteen tumors of the nasopharyngeal region were analyzed for the presence of Epstein-Barr virus-specific DNA by DNA-cRNA hybridization. These data were compared to the histology of the respective tumors and the seroreactivity of the tumor-bearing patient against EBV-related antigens. With one exception, all tumor pieces containing nasopharyngeal carcinoma cells hybridized significantly with EBV-cRNA. Tumors of predominantly epithelial morphology annealed in the highest range. In situ-hybridizations of freeze sections from a tumor containing almost equal amounts of tumor cells and lymphocytes revealed hybridizing DNA within nuclei of non-lymphoid cells. Although these data do not exclude the presence of EBV-DNA within lymphoid cells, they clearly demonstrate that in nasopharyngeal carcinomas the vast majority of EBV-specific DNA rests within non-lymphoid cells.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalMedical Microbiology and Immunology
Volume161
Issue number1
DOIs
StatePublished - Mar 1975
Externally publishedYes

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