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Associations between NBS1 polymorphisms, haplotypes and smoking-related cancers

  • Sungshim L. Park
  • , Delara Bastani
  • , Binh Y. Goldstein
  • , Shen Chih Chang
  • , Wendy Cozen
  • , Lin Cai
  • , Carlos Cordon-Cardo
  • , Baoguo Ding
  • , Sander Greenland
  • , Na He
  • , Shehnaz K. Hussain
  • , Qingwu Jiang
  • , Yuan Chin A. Lee
  • , Simin Liu
  • , Ming Lan Lu
  • , Thomas M. Mack
  • , Jenny T. Mao
  • , Hal Morgenstern
  • , Li Na Mu
  • , Sam S. Oh
  • Allan Pantuck, Jeanette C. Papp, Jianyu Rao, Victor E. Reuter, Donald P. Tashkin, Hua Wang, Nai Chieh Y. You, Shun Zhang Yu, Jin Kou Zhao, Zuo Feng Zhang

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Constituents of tobacco smoke can cause DNA double-strand breaks (DSBs), leading to tumorigenesis. The NBS1 gene product is a vital component in DSB detection and repair, thus genetic variations may influence cancer development. We examined the associations between NBS1 polymorphisms and haplotypes and newly incident smoking-related cancers in three case-control studies (Los Angeles: 611 lung and 601 upper aero-digestive tract (UADT) cancer cases and 1040 controls; Memorial Sloan-Kettering Cancer Center: 227 bladder cancer cases and 211 controls and Taixing, China: 218 esophagus, 206 stomach, 204 liver cancer cases and 415 controls). rs1061302 was associated with cancers of the lung [adjusted odds ratio (ORadj) = 1.6, 95% confidence interval (CI): 1.2, 2.4], larynx (ORadj = 0.56, 95% CI: 0.32, 0.97) and liver (ORadj = 1.7, 95% CI: 1.0, 2.9). Additionally, positive associations were found for rs709816 with bladder cancer (ORadj = 4.2, 95% CI: 1.4, 12) and rs1063054 with lung cancer (ORadj = 1.6, 95% CI: 1.0, 2.3). Some associations in lung and stomach cancers varied with smoking status. CAC haplotype was positively associated with smoking-related cancers: lung (ORadj = 1.7, 95% CI: 1.1, 2.9) and UADT (ORadj = 2.0, 95% CI: 1.1, 3.7), specifically, oropharynx (ORadj = 2.1, 95% CI: 1.0, 4.2) and larynx (ORadj = 4.8, 95% CI: 1.7, 14). Bayesian falsediscovery probabilities were calculated to assess Type I error. It appears that NBS1 polymorphisms and haplotypes may be associated with smoking-related cancers and that these associations may differ by smoking status. Our findings also suggest that single-nucleotide polymorphisms located in the binding region of the MRE-RAD50-NBS1 complex or microRNA targeted pathways may influence tumor development. These hypotheses should be further examined in functional studies.

Original languageEnglish
Pages (from-to)1264-1271
Number of pages8
JournalCarcinogenesis
Volume31
Issue number7
DOIs
StatePublished - 17 May 2010
Externally publishedYes

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