Assignment and ordering of twenty-three unique NotI-Linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3

L. Fedorova; M. Kost-Alimova; R. Z. Gizatullin; A. Alimov; V. I. Zabarovska; A. Szeles; A. I. Protopopov; N. V. Vorobieva; V. I. Kashuba; G. Klein; A. V. Zelenin; D. Sheer; E. R. Zabarovsky

doi:10.1159/000484744

Assignment and ordering of twenty-three unique NotI-Linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3

L. Fedorova, M. Kost-Alimova, R. Z. Gizatullin, A. Alimov, V. I. Zabarovska, A. Szeles, A. I. Protopopov, N. V. Vorobieva, V. I. Kashuba, G. Klein, A. V. Zelenin, D. Sheer, E. R. Zabarovsky

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Twenty-three unique NotI-linking clones, mainly isolated from the NRL1 library, were mapped and ordered by fluorescence in situ hybridization to human chromosome 3. All these clones were partially sequenced around the NotI sites and thus represent sequence-tagged sites. The EMBL nucleotide database was then searched with sequences from the NotI-linking clones using the FASTA program. This search revealed that the NRL-090 clone (at 3q24) contains the gene encoding human guanosine 5'-monophosphate synthetase (GMPS-PEN). To our knowledge, this is the first localization of this gene. Clone NL1-320 (at 3p21.3) contains a gene encoding arginine tRNA (97.3% identity in 73 bp), while clones NRL-063, NRL-097 and NRL-143 contain expressed sequences with unknown functions. Other clones displayed 60-85% similarities to cDNAs, CpG islands and other genes.

Original language	English
Pages (from-to)	110-116
Number of pages	7
Journal	European Journal of Human Genetics
Volume	5
Issue number	2
DOIs	https://doi.org/10.1159/000484744
State	Published - 1997
Externally published	Yes

Keywords

Fluorescence in situ hybridization
Human chromosome 3
NotI-linking clones

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10.1159/000484744

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Fedorova, L., Kost-Alimova, M., Gizatullin, R. Z., Alimov, A., Zabarovska, V. I., Szeles, A., Protopopov, A. I., Vorobieva, N. V., Kashuba, V. I., Klein, G., Zelenin, A. V., Sheer, D., & Zabarovsky, E. R. (1997). Assignment and ordering of twenty-three unique NotI-Linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3. European Journal of Human Genetics, 5(2), 110-116. https://doi.org/10.1159/000484744

@article{8a7b1eaf86ed4b80b519c805252f22d8,

title = "Assignment and ordering of twenty-three unique NotI-Linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3",

abstract = "Twenty-three unique NotI-linking clones, mainly isolated from the NRL1 library, were mapped and ordered by fluorescence in situ hybridization to human chromosome 3. All these clones were partially sequenced around the NotI sites and thus represent sequence-tagged sites. The EMBL nucleotide database was then searched with sequences from the NotI-linking clones using the FASTA program. This search revealed that the NRL-090 clone (at 3q24) contains the gene encoding human guanosine 5'-monophosphate synthetase (GMPS-PEN). To our knowledge, this is the first localization of this gene. Clone NL1-320 (at 3p21.3) contains a gene encoding arginine tRNA (97.3% identity in 73 bp), while clones NRL-063, NRL-097 and NRL-143 contain expressed sequences with unknown functions. Other clones displayed 60-85% similarities to cDNAs, CpG islands and other genes.",

keywords = "Fluorescence in situ hybridization, Human chromosome 3, NotI-linking clones",

author = "L. Fedorova and M. Kost-Alimova and Gizatullin, {R. Z.} and A. Alimov and Zabarovska, {V. I.} and A. Szeles and Protopopov, {A. I.} and Vorobieva, {N. V.} and Kashuba, {V. I.} and G. Klein and Zelenin, {A. V.} and D. Sheer and Zabarovsky, {E. R.}",

year = "1997",

doi = "10.1159/000484744",

language = "English",

volume = "5",

pages = "110--116",

journal = "European Journal of Human Genetics",

issn = "1018-4813",

publisher = "Nature Publishing Group",

number = "2",

}

Fedorova, L, Kost-Alimova, M, Gizatullin, RZ, Alimov, A, Zabarovska, VI, Szeles, A, Protopopov, AI, Vorobieva, NV, Kashuba, VI, Klein, G, Zelenin, AV, Sheer, D & Zabarovsky, ER 1997, 'Assignment and ordering of twenty-three unique NotI-Linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3', European Journal of Human Genetics, vol. 5, no. 2, pp. 110-116. https://doi.org/10.1159/000484744

Assignment and ordering of twenty-three unique NotI-Linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3. / Fedorova, L.; Kost-Alimova, M.; Gizatullin, R. Z. et al.
In: European Journal of Human Genetics, Vol. 5, No. 2, 1997, p. 110-116.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Assignment and ordering of twenty-three unique NotI-Linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3

AU - Fedorova, L.

AU - Kost-Alimova, M.

AU - Gizatullin, R. Z.

AU - Alimov, A.

AU - Zabarovska, V. I.

AU - Szeles, A.

AU - Protopopov, A. I.

AU - Vorobieva, N. V.

AU - Kashuba, V. I.

AU - Klein, G.

AU - Zelenin, A. V.

AU - Sheer, D.

AU - Zabarovsky, E. R.

PY - 1997

Y1 - 1997

N2 - Twenty-three unique NotI-linking clones, mainly isolated from the NRL1 library, were mapped and ordered by fluorescence in situ hybridization to human chromosome 3. All these clones were partially sequenced around the NotI sites and thus represent sequence-tagged sites. The EMBL nucleotide database was then searched with sequences from the NotI-linking clones using the FASTA program. This search revealed that the NRL-090 clone (at 3q24) contains the gene encoding human guanosine 5'-monophosphate synthetase (GMPS-PEN). To our knowledge, this is the first localization of this gene. Clone NL1-320 (at 3p21.3) contains a gene encoding arginine tRNA (97.3% identity in 73 bp), while clones NRL-063, NRL-097 and NRL-143 contain expressed sequences with unknown functions. Other clones displayed 60-85% similarities to cDNAs, CpG islands and other genes.

AB - Twenty-three unique NotI-linking clones, mainly isolated from the NRL1 library, were mapped and ordered by fluorescence in situ hybridization to human chromosome 3. All these clones were partially sequenced around the NotI sites and thus represent sequence-tagged sites. The EMBL nucleotide database was then searched with sequences from the NotI-linking clones using the FASTA program. This search revealed that the NRL-090 clone (at 3q24) contains the gene encoding human guanosine 5'-monophosphate synthetase (GMPS-PEN). To our knowledge, this is the first localization of this gene. Clone NL1-320 (at 3p21.3) contains a gene encoding arginine tRNA (97.3% identity in 73 bp), while clones NRL-063, NRL-097 and NRL-143 contain expressed sequences with unknown functions. Other clones displayed 60-85% similarities to cDNAs, CpG islands and other genes.

KW - Fluorescence in situ hybridization

KW - Human chromosome 3

KW - NotI-linking clones

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DO - 10.1159/000484744

M3 - Article

C2 - 9195163

AN - SCOPUS:12644298665

SN - 1018-4813

VL - 5

SP - 110

EP - 116

JO - European Journal of Human Genetics

JF - European Journal of Human Genetics

IS - 2

ER -

Assignment and ordering of twenty-three unique NotI-Linking clones containing expressed genes including the guanosine 5'-monophosphate synthetase gene to human chromosome 3

Abstract

Keywords

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