TY - JOUR
T1 - Assessment of cardiovascular fibrosis using novel fluorescent probes
AU - Chen, Jiqiu
AU - Lee, Seung Koo
AU - Abd-Elgaliel, Wael R.
AU - Liang, Lifan
AU - Galende, Elisa Yaniz
AU - Hajjar, Roger J.
AU - Tung, Ching Hsuan
PY - 2011
Y1 - 2011
N2 - Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed pathological characteristics of cardiovascular fibrosis could be identified clearly in 2 hours. This staining pair requires only simple staining and brief washing, generating less than 10 ml of waste. The image information collected by this novel fluorescent staining pair is compatible with it collected by the traditional Masson's Trichrome and Picrosirius Red staining which are widely used to stain cardiovascular fibrosis and isolated cells.
AB - Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed pathological characteristics of cardiovascular fibrosis could be identified clearly in 2 hours. This staining pair requires only simple staining and brief washing, generating less than 10 ml of waste. The image information collected by this novel fluorescent staining pair is compatible with it collected by the traditional Masson's Trichrome and Picrosirius Red staining which are widely used to stain cardiovascular fibrosis and isolated cells.
UR - http://www.scopus.com/inward/record.url?scp=79955377033&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0019097
DO - 10.1371/journal.pone.0019097
M3 - Article
C2 - 21533060
AN - SCOPUS:79955377033
SN - 1932-6203
VL - 6
JO - PLoS ONE
JF - PLoS ONE
IS - 4
M1 - e19097
ER -