Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples

Weihua Huang; Guiqing Wang; Henry Lin; Jian Zhuge; Sheila M. Nolan; Eric Vail; Nevenka Dimitrova; John T. Fallon

doi:10.1016/j.diagmicrobio.2016.01.013

Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples

Weihua Huang, Guiqing Wang, Henry Lin, Jian Zhuge, Sheila M. Nolan, Eric Vail, Nevenka Dimitrova, John T. Fallon

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.

Original language	English
Pages (from-to)	26-29
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	85
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2016.01.013
State	Published - 1 May 2016
Externally published	Yes

Keywords

Bioinformatics
Diagnostic sensitivity
Diagnostic specificity
Enterovirus D68
Next-generation sequencing

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10.1016/j.diagmicrobio.2016.01.013

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title = "Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples",

abstract = "We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.",

keywords = "Bioinformatics, Diagnostic sensitivity, Diagnostic specificity, Enterovirus D68, Next-generation sequencing",

author = "Weihua Huang and Guiqing Wang and Henry Lin and Jian Zhuge and Nolan, \{Sheila M.\} and Eric Vail and Nevenka Dimitrova and Fallon, \{John T.\}",

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doi = "10.1016/j.diagmicrobio.2016.01.013",

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AU - Huang, Weihua

AU - Wang, Guiqing

AU - Lin, Henry

AU - Zhuge, Jian

AU - Nolan, Sheila M.

AU - Vail, Eric

AU - Dimitrova, Nevenka

AU - Fallon, John T.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.

AB - We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.

KW - Bioinformatics

KW - Diagnostic sensitivity

KW - Diagnostic specificity

KW - Enterovirus D68

KW - Next-generation sequencing

UR - https://www.scopus.com/pages/publications/84959867866

U2 - 10.1016/j.diagmicrobio.2016.01.013

DO - 10.1016/j.diagmicrobio.2016.01.013

M3 - Article

C2 - 26971640

AN - SCOPUS:84959867866

SN - 0732-8893

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IS - 1

ER -

Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples

Abstract

Keywords

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