Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples

Weihua Huang; Guiqing Wang; Henry Lin; Jian Zhuge; Sheila M. Nolan; Eric Vail; Nevenka Dimitrova; John T. Fallon

doi:10.1016/j.diagmicrobio.2016.01.013

Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples

Weihua Huang
, Guiqing Wang
, Henry Lin
, Jian Zhuge
, Sheila M. Nolan
, Eric Vail
, Nevenka Dimitrova
, John T. Fallon

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.

Original language	English
Pages (from-to)	26-29
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	85
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2016.01.013
State	Published - 1 May 2016
Externally published	Yes

Keywords

Bioinformatics
Diagnostic sensitivity
Diagnostic specificity
Enterovirus D68
Next-generation sequencing

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10.1016/j.diagmicrobio.2016.01.013

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title = "Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples",

abstract = "We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.",

keywords = "Bioinformatics, Diagnostic sensitivity, Diagnostic specificity, Enterovirus D68, Next-generation sequencing",

author = "Weihua Huang and Guiqing Wang and Henry Lin and Jian Zhuge and Nolan, \{Sheila M.\} and Eric Vail and Nevenka Dimitrova and Fallon, \{John T.\}",

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doi = "10.1016/j.diagmicrobio.2016.01.013",

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AU - Huang, Weihua

AU - Wang, Guiqing

AU - Lin, Henry

AU - Zhuge, Jian

AU - Nolan, Sheila M.

AU - Vail, Eric

AU - Dimitrova, Nevenka

AU - Fallon, John T.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.

AB - We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.

KW - Bioinformatics

KW - Diagnostic sensitivity

KW - Diagnostic specificity

KW - Enterovirus D68

KW - Next-generation sequencing

UR - https://www.scopus.com/pages/publications/84959867866

U2 - 10.1016/j.diagmicrobio.2016.01.013

DO - 10.1016/j.diagmicrobio.2016.01.013

M3 - Article

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AN - SCOPUS:84959867866

SN - 0732-8893

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JF - Diagnostic Microbiology and Infectious Disease

IS - 1

ER -

Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples

Abstract

Keywords

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