Assays for cytokines using aptamers

Jeffrey W. Guthrie; Camille L.A. Hamula; Hongquan Zhang; X. Chris Le

doi:10.1016/j.ymeth.2006.01.001

Assays for cytokines using aptamers

Jeffrey W. Guthrie
, Camille L.A. Hamula
, Hongquan Zhang
, X. Chris Le

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Aptamers are short nucleic acid sequences that are used as ligands to bind their targets with high affinity. They are generated via the combinatorial chemistry procedure systematic evolution of ligands by exponential enrichment (SELEX). Aptamers have shown much promise towards detection of a variety of protein targets, including cytokines. Specifically, for the determination of cytokines and growth factors, several assays making use of aptamers have been developed, including aptamer-based enzyme-linked immunosorbent assays, antibody-linked oligonucleotide assay, fluorescence (anisotropy and resonance energy transfer) assays, and proximity ligation assays. In this article, the concept of aptamer selection using SELEX and the assay formats using aptamers for the detection of cytokines are discussed.

Original language	English
Pages (from-to)	324-330
Number of pages	7
Journal	Methods
Volume	38
Issue number	4
DOIs	https://doi.org/10.1016/j.ymeth.2006.01.001
State	Published - Apr 2006
Externally published	Yes

Keywords

Aptamers
Combinatorial chemistry
Cytokines
In vitro selection
Proteins1
SELEX

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10.1016/j.ymeth.2006.01.001

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title = "Assays for cytokines using aptamers",

abstract = "Aptamers are short nucleic acid sequences that are used as ligands to bind their targets with high affinity. They are generated via the combinatorial chemistry procedure systematic evolution of ligands by exponential enrichment (SELEX). Aptamers have shown much promise towards detection of a variety of protein targets, including cytokines. Specifically, for the determination of cytokines and growth factors, several assays making use of aptamers have been developed, including aptamer-based enzyme-linked immunosorbent assays, antibody-linked oligonucleotide assay, fluorescence (anisotropy and resonance energy transfer) assays, and proximity ligation assays. In this article, the concept of aptamer selection using SELEX and the assay formats using aptamers for the detection of cytokines are discussed.",

keywords = "Aptamers, Combinatorial chemistry, Cytokines, In vitro selection, Proteins1, SELEX",

author = "Guthrie, \{Jeffrey W.\} and Hamula, \{Camille L.A.\} and Hongquan Zhang and Le, \{X. Chris\}",

note = "Funding Information: The authors acknowledge the financial support from the Natural Sciences and Engineering Research Council of Canada, the Canada Research Chair Program, an Alberta Ingenuity Postdoctoral Fellowship (to J.W.G.), an Alberta Heritage Foundation for Medical Research Studentship (to C.L.A.H.), and a Graduate Assistantship from the University of Alberta (to H.Z.).",

year = "2006",

month = apr,

doi = "10.1016/j.ymeth.2006.01.001",

language = "English",

volume = "38",

pages = "324--330",

journal = "Methods",

issn = "1046-2023",

publisher = "Academic Press Inc.",

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T1 - Assays for cytokines using aptamers

AU - Guthrie, Jeffrey W.

AU - Hamula, Camille L.A.

AU - Zhang, Hongquan

AU - Le, X. Chris

N1 - Funding Information: The authors acknowledge the financial support from the Natural Sciences and Engineering Research Council of Canada, the Canada Research Chair Program, an Alberta Ingenuity Postdoctoral Fellowship (to J.W.G.), an Alberta Heritage Foundation for Medical Research Studentship (to C.L.A.H.), and a Graduate Assistantship from the University of Alberta (to H.Z.).

PY - 2006/4

Y1 - 2006/4

N2 - Aptamers are short nucleic acid sequences that are used as ligands to bind their targets with high affinity. They are generated via the combinatorial chemistry procedure systematic evolution of ligands by exponential enrichment (SELEX). Aptamers have shown much promise towards detection of a variety of protein targets, including cytokines. Specifically, for the determination of cytokines and growth factors, several assays making use of aptamers have been developed, including aptamer-based enzyme-linked immunosorbent assays, antibody-linked oligonucleotide assay, fluorescence (anisotropy and resonance energy transfer) assays, and proximity ligation assays. In this article, the concept of aptamer selection using SELEX and the assay formats using aptamers for the detection of cytokines are discussed.

AB - Aptamers are short nucleic acid sequences that are used as ligands to bind their targets with high affinity. They are generated via the combinatorial chemistry procedure systematic evolution of ligands by exponential enrichment (SELEX). Aptamers have shown much promise towards detection of a variety of protein targets, including cytokines. Specifically, for the determination of cytokines and growth factors, several assays making use of aptamers have been developed, including aptamer-based enzyme-linked immunosorbent assays, antibody-linked oligonucleotide assay, fluorescence (anisotropy and resonance energy transfer) assays, and proximity ligation assays. In this article, the concept of aptamer selection using SELEX and the assay formats using aptamers for the detection of cytokines are discussed.

KW - Aptamers

KW - Combinatorial chemistry

KW - Cytokines

KW - In vitro selection

KW - Proteins1

KW - SELEX

UR - https://www.scopus.com/pages/publications/33644945214

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DO - 10.1016/j.ymeth.2006.01.001

M3 - Article

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AN - SCOPUS:33644945214

SN - 1046-2023

VL - 38

SP - 324

EP - 330

JO - Methods

JF - Methods

IS - 4

ER -

Assays for cytokines using aptamers

Abstract

Keywords

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