Aspiration flow cytometry of the testes in the evaluation of spermatogenesis in the infertile male

D. G. Kaufman, H. M. Nagler

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Deoxyribonucleic acid (DNA) flow cytometry of testicular tissue has been demonstrated to be a quantitative means of assessing spermatogenesis. This study evaluates testes aspirates and DNA flow cytometry in the evaluation of the infertile male. Testicular tissue obtained from 12 men who underwent bilateral orchiectomy for prostate cancer (group 1) were examined by both flow cytometry and standard histologic technique to assess the correlation between these two modalities. Thirteen men evaluated for infertility (group 2) and requiring histologic evaluation of spermatogenesis underwent both open biopsy and fine needle aspiration of their testes. Histology was independently examined and grouped according to standard nomenclature. Flow cytometric analysis revealed characteristic patterns in the relative numbers of haploid (1C), diploid (2C), and tetraploid (4C) cells. These patterns correlated reproducibly with the histologic diagnoses. DNA flow cytometry of testicular aspirates provides a rapid and reliable quantitative means of assessing spermatogenesis.

Original languageEnglish
Pages (from-to)287-291
Number of pages5
JournalFertility and Sterility
Volume48
Issue number2
DOIs
StatePublished - 1987
Externally publishedYes

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