TY - JOUR
T1 - Applying stem cells and CRISPR engineering to uncover the etiology of schizophrenia
AU - Michael Deans, Peter James
AU - Brennand, Kristen J.
N1 - Funding Information:
This work was partially supported by National Institute of Health ( NIH ) grants R56 MH101454 (K.J.B.), R01 MH106056 (K.J.B.), and R01 MH109897 (K.J.B.).
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/8
Y1 - 2021/8
N2 - Schizophrenia is a highly heritable, polygenic disorder. A growing list of common genetic variants have been associated with schizophrenia; there is a clear need to understand the role of these risk factors in the etiology of disease. The majority of these variants occur in noncoding regions of the genome and are thought to regulate the expression of one or more genes in a cell type–specific fashion. Recent advances in stem cell biology and molecular genetics have resulted in two invaluable advances: hiPSC technology makes possible the generation of donor-specific disease-relevant neural cell types, whereas CRISPR-based techniques can be applied to manipulate individual variants and/or their gene targets. New multiplexed gene manipulation and CRISPR screening techniques show great promise toward dissecting the complex interactions between the myriad disease-associated variants. This review outlines key advances in hiPSC and CRISPR technology, describing their applications and future potential in the field of schizophrenia research.
AB - Schizophrenia is a highly heritable, polygenic disorder. A growing list of common genetic variants have been associated with schizophrenia; there is a clear need to understand the role of these risk factors in the etiology of disease. The majority of these variants occur in noncoding regions of the genome and are thought to regulate the expression of one or more genes in a cell type–specific fashion. Recent advances in stem cell biology and molecular genetics have resulted in two invaluable advances: hiPSC technology makes possible the generation of donor-specific disease-relevant neural cell types, whereas CRISPR-based techniques can be applied to manipulate individual variants and/or their gene targets. New multiplexed gene manipulation and CRISPR screening techniques show great promise toward dissecting the complex interactions between the myriad disease-associated variants. This review outlines key advances in hiPSC and CRISPR technology, describing their applications and future potential in the field of schizophrenia research.
UR - http://www.scopus.com/inward/record.url?scp=85105815002&partnerID=8YFLogxK
U2 - 10.1016/j.conb.2021.04.003
DO - 10.1016/j.conb.2021.04.003
M3 - Review article
C2 - 34010781
AN - SCOPUS:85105815002
SN - 0959-4388
VL - 69
SP - 193
EP - 201
JO - Current Opinion in Neurobiology
JF - Current Opinion in Neurobiology
ER -