Application of in situ hybridization probes for MLH-1 and MSH-2 in tissue microarrays of paraffin-embedded malignant melanomas: Correlation with immunohistochemistry and tumor stage

Monika Korabiowska, Carlos Cordon-Cardo, Fredericke Jaenckel, Jerzy Stachura, Gösta Fischer, Ulrich Brinck

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Defects in DNA mismatch-repair genes MLH1 and MSH2 reported primarily in hereditary nonpolyposis colorectal carcinoma are present in many sporadic tumors, including malignant melanomas. The main aim of this study was to investigate the expression of these genes in malignant melanomas in relation to tumor stage. An experiment was performed on paraffin-embedded tissue microarrays of malignant melanomas applying in situ hybridization with probes produced by our research group and immunohistochemical techniques. In situ hybridization demonstrated MLH1 expression in 45 of 59 melanomas and MSH2 expression in 51 of 59 melanomas. Immunohistochemistry detected MLH1 expression in 46 of 59 melanomas and MSH2 expression in 50 of 59 melanomas. Down-regulation of expression of both DNA mismatch repair genes in malignant melanomas was observed. The findings obtained by in situ hybridization and immunohistochemistry correlated significantly. Our study demonstrates the suitability of in situ hybridization with MLH1 and MSH2 probes for paraffin-embedded tissue. Tissue microarrays can be used successfully in both in situ hybridization and immunohistochemistry to analyze the expression of DNA mismatch-repair genes.

Original languageEnglish
Pages (from-to)1543-1548
Number of pages6
JournalHuman Pathology
Volume35
Issue number12
DOIs
StatePublished - Dec 2004
Externally publishedYes

Keywords

  • DNA
  • HNPCC
  • HRP
  • PCR
  • RT-PCR
  • SDS
  • cutaneous tumor
  • hereditary nonpolyposis colorectal carcinoma
  • horseradish peroxidase
  • mismatch repair
  • pigmented skin lesion
  • polymerase chain reaction
  • reverse-transcription polymerase chain reaction
  • sodium dodecyl sulfate

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